LAB: BROAD SPECTRUM COMPATIBILITY TEST

Question 1

series of procedures designed to ensure the safety of blood for transfusion

Answer 1

compatibility testing

Question 2

performed prior to the transfusion of blood components containing red blood cells

Answer 2

crossmatching

Question 3

2 parts of crossmatching

Answer 3

major crossmatch
minor crossmatch

Question 4

what does major crossmatch consists

Answer 4

mixing the px serum with donor red cells (PS-DR)

Question 5

what does minor crossmatch consists

Answer 5

mixing the donor’s serum with the px red cells (PR-DS)

Question 6

autocontrol consists of

Answer 6

px red cells and px serum

Question 7

M label stands for

Answer 7

major crossmatch

Question 8

MA label stands for

Answer 8

major crossmatch w/ albumin

Question 9

N label stands for

Answer 9

minor crossmatch

Question 10

NA label stands for

Answer 10

minor crossmatch w/ albumin

Question 11

immediate saline spin is aka

Answer 11

protein/albumin/room temperature phase

Question 12

how many seconds are the tubes centrifugated in immediate spin

Answer 12

15 s

Question 13

if no agglutination or hemolysis is observed, what tubes will proceed to the thermo phase

Answer 13

MA and NA

Question 14

what reagent can be used instead of 22% bovine serum albumin if the tubes proceeding to the next step will be incubated for 10 mins

Answer 14

LISS

Question 15

incubation phase is aka

Answer 15

thermo phase

Question 16

MA and NA tubes are incubated for how many minutes

Answer 16

30 minutes

Question 17

how many minutes are the tubes incubated if LISS was used

Answer 17

10 minutes

Question 18

proceed to AHG phase if

Answer 18

no agglutination or hemolysis is observed in MA and NA tubes

Question 19

at what phase is check cells reagent added

Answer 19

AHG phase

Question 20

how many times are the cells washed in AHG phase

Answer 20

3 times

Question 21

how many drops of AHG sera are added

Answer 21

2 drops

Question 22

in AHG phase, tubes are centrifuged for how many seconds

Answer 22

15 seconds

Question 23

how many drop of check cells are added

Answer 23

1 drop

Question 24

interpret:
no agglutination or hemolysis in all tubes

Answer 24

compatible

Question 25

interpret: agglutination or hemolysis in any tubes

Answer 25

incompatible

Question 26

Blood is compatible in major crossmatch but incompatible in minor crossmatch. What should be done?

Answer 26

Blood can be transfused but with caution

Question 27

contents of M tube

Answer 27

2 drops px serum, 1 drop 2-5% suspension of donor's cells

Question 28

contents of MA tube

Answer 28

2 drops px serum, 1 drop 2-5% suspension of donor's cells 2 drops of 22% BSA

Question 29

contents of N tube

Answer 29

2 drops of donor's serum 1 drop of 2-5% suspension of px cells

Question 30

contents of NA tube

Answer 30

2 drops of donor's serum 1 drop of 2-5% suspension of px cells 2 drops of BSA