Lab Compre (RVSP) Flashcards
Veins for venipuncture
Cephalic vein
Basilic vein
Median cubital vein
Ideal puncture depth
2-3 mm
Inches above the site the tourniquet is applied
3-4 in
Angle in venipuncture
15°
Ideal venipuncture depth
0.5 - 1.0 cm
Order of draw accdg to color
Yellow Light blue Red Green Lavender Gray
Oder of draw accdg to function
Blood culture Coagulation Serology/BB Plasma chemistry Blood cell counting Glucose
Order of draw accdg to additives
ACD & SPS Sodium citrate None Sodium/lithium heparin K2EDTA Sodium fluoride-potassium oxalate
Additive; inhibits thrombin formation to prevent clotting
Sodium heparin/Lithium heparin
Needle angle for arterial puncture
35-40°
Most popular method for smear preparatiom
Two-slide/Wedge method
Angle of spreader slide in smear prep
30-45°
4 criterion for a good smear
- even transition from thick to thin area
- must cover 2/3 or 3/4 of the slide
- smooth and even surface, free from ridges, waves, or holes
- feathery edge
Other mtds for smear prep
- Two-cover slip/Ehrlich’s Two-cover glass method
- Spinner’s method
- Automated smear preparation
Instrument used for Spinner’s mtd of smar prep
Hemaspinner
Method for staining blood smear
Staining jar/Dip method
Stains in the Staining jar/dip method
Methanol
Eosin
Methylene blue
Buffer solution/aged distilled water
4 mtds for differential counting
Four-field meander
Two-field
Exaggerated battlement
Strip differential
6 stations in the Hemastainer Automatic Slide Stainer
- Methanol
- Wright’s or Wright-Giemsa stain
- Stain-Buffer mixture (Wright’s or Wright-Giemsa + Phosphate buffer)
- Deionized water
- Phosphate buffer
- Warm air
Mtd of staining; blood smear is placed on a rack positioned in a dish
Staining Dish Mtd
Contained in the Stain-Pak
Stain
Buffer
Rinse solution
Staining mtd; uses Stain-Pak
- Hema-Tek 1000 Slide Stainer
- Hema-Tek 2000 Slide Stainer
Stain for diff ct; contains methylene blue or its oxidative product like Azure B; also contains Eosin B or Eosin Y
Romanowsky Stains
Most satisfactory type of Romanowsky stain for general routine hematologic studies
Wright’s stain
