RBC, WBC, Counting Chamber (RVSP)

Question 0

7 characteristics of a good RBC diluting fluid

Answer 0

• isotonic solution
• has a high specific gravity
• easy to prepare
• cheap
• good preservative
• has a buffer action
• must not initiate the growth of molds

Question 1

The red count is

Answer 1

Number of red cells in 1 cu mm of blood

Question 2

Dilution in RBC count

Answer 2

1:200

Question 3

Number of drops discarded in the RBC pipet

Answer 3

First 5-6 drops

Question 4

Angle for charging in RBC ct

Answer 4

30-35°

Question 5

Presence of fluid on the moats of the counting chamber

Answer 5

Overcharging

Question 6

Failure to cover the entire ruled area of the counting chamber

Answer 6

Undercharging

Question 7

Number of mins to allow the RBCs to settle

Answer 7

5-10 mins

Question 8

Objective used to initially focus the ruled area in RBC counting

Answer 8

LPO

Question 9

Objective used for actual red cell counting

Answer 9

HPO

Question 10

Cell difference bet. 2 squares in RBC counting

Answer 10

20 or less

Question 11

Best RBC diluting fluid

Answer 11

Dacie’s fluid/Formol citrate

Question 12

Composition of Dacie’s fluid

Answer 12

40% soln of formaldehyde

3% W/V trisodium citrate

Question 13

RBC diluting fluid; allows the growth of yeasts and produces clumping of cells in patients suffering from liver cirrhosis

Answer 13

Hayem’s

Question 14

Composition of Hayem’s fluid

Answer 14

Mercuric chloride
Sodium sulfate (crystalline/anhydrous)
Sodium chloride
Distilled water

Question 15

RBC diluting fluid; prevents rouleaux formation, precipitates proteins in cases of hemoglobinuria and hyperglobulinemia

Answer 15

Gower’s

Question 16

Composition of Gower’s fluid

Answer 16

Glacial acetic acid
Sodium sulfate (crystalline)
Distilled water

Sodium chloride
Sodium sulfate
Glycerine
Distilled water

Question 17

RBC diluting fluid; supports the growth of fungi

Answer 17

Toisson’s

Question 18

Composition of Toisson’s fluid

Answer 18

Sodium chloride
Sodium sulfate
Glycerine
Methyl violet
Distilled water

Question 19

Composition of Bethell’s fluid (RBC diluting fluid)

Answer 19

Sodium sulfate
Sodium chloride
Glycerine
Sodium merthiolate
Distilled water

Question 20

RBC diluting fluid; used in cases of emergency

Answer 20

Normal saline solution (NSS) or Physiologic Saline solution

Question 21

Composition of NSS

Answer 21

Sodium chloride

Distilled water

Question 22

Composition of 3.8% sodium citrate (RBC diluting fluid)

Answer 22

Sodium citrate

Distilled water

Question 23

First model of Coulter Counter

Answer 23

Coulter Counter Model S

Question 24

Coulter counter; + RDW

Answer 24

Coulter counter model S Plus

Question 25

Coulter counter; histogram differential

Answer 25

Coulter Counter Model S Plus IV

Question 26

Coulter counter; 90 Sx/hr

Answer 26

Coulter Counter Model S Plus II

Question 27

Coulter counter; 115 Sx/hr

Answer 27

Coulter Counter Model S Plus III

Question 28

Coulter counter; blood sample volume reduced to 100 uL instead of 1 mL

Answer 28

Coulter Counter Model S Plus IV

Question 29

Coulter counter; automated ca piercer

Answer 29

Coulter Counter Model S Plus V

Question 30

Coulter Counter; autosampler with 2 rotating trays that can hold 32 tubes each

Answer 30

Coulter Counter Model S Plus VI

Question 31

Coulter Counter; capable of accepting and mixing 144 Sx at one time in preparation for Sx identification, aspiration, and analysis

Answer 31

Coulter Counter Model S Plus STKR

Question 32

Technicon; 60 Sx/hr

Answer 32

Technicon H-1

Question 33

Technicon; 100 Sx/hr

Answer 33

Technicon H-2

Question 34

The white cell count is

Answer 34

The number of white cells in 1 cumm of blood

Question 35

Characteristics of a good WBC diluting fluid

Answer 35

- hypotonic solution - easily prepared - cheap - readily available - good preservative

Question 36

Dilution for WBC ct

Answer 36

1:20

Question 37

Number of drops discarded from the WBC pipet

Answer 37

2-3 drops

Question 38

Objective used for WBC counting

Answer 38

LPO

Question 39

Cell diference between squares in WBC count

Answer 39

12 cells or less

Question 40

Composition of 1-3% Acetic acid with Gentian violet (WBC diluting fluid)

Answer 40

Glacial acetic acid Gentian violet Distilled water

Question 41

Composition of 1% HCl (WBC diluting fluid)

Answer 41

Hydrochloric acid | Distilled water

Question 42

Composition of Tuerk's fluid (WBC diluting fluid)

Answer 42

Glacial acetic acid Methyl violet Distilled water