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lab exam 1 Flashcards

1
Q

what goes in biohazard container

A

live container, liquid/solid culture,gloves

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2
Q

what goes in the general trash

A

paper towels, Kimwipes, paper and gloves

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3
Q

what goes in plastic pipettes

A

pl;astic loops, plastic needles

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4
Q

What is the objective of Aspetic Teechnique?

A

transfer of bacteria from one medium to another without introducing contaminants

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5
Q

What is the objective of subculturing

A

the transfer of microorganism from one medium to another for fresh growth

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6
Q

what is a pure culture

A

population of a single species

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7
Q

what is a mixed culture

A

population containing two or more species

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8
Q

streak plate is also called

A

streaking for singles

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9
Q

hwo do you streak plate

A

essentially a dilution, created by a loopful of culture over the surface of an agar plate

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10
Q

how do you spread plate

A

microorganism spread evenly throughout the plate

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11
Q

describe pseudomonas aeruginosa

A

ROD, can be pigmented, major cause of lung infection with cystic fibrous

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12
Q

describe staph, aureus

A

COCCUS, harmless skin bacteria to MRSA(pathogen)

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13
Q

Describe bacillus cereus

A

ROD, spore former, soil bacteria can cause food poisoning

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14
Q

which bacteria can be milky white to golden

A

S.aureus

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15
Q

purpose of bacterial smear

A

can be stained so as to better view the bacteria

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16
Q

what does a stain consist of

A

benzene ring, a chromophore, auxochrome

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17
Q

what does a chromophore do

A

imparts color

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18
Q

what does auxochrome

A

ionizes the chromogen

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19
Q

is a basic stain positively or negatively charged

A

positively charged

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20
Q

is a acid stain positively or negatively charged

A

negatively charges

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21
Q

examples of basic stains

A

methylene blue, carbon fuschin and crystal violet

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22
Q

correct exposure for methylene blue

A

1-2 min

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23
Q

correct exposure for crystal violet

A

20-60 secs

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24
Q

correct exposure for carbol fuchsin

A

15-30 sec

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25
examples of acidic stain
india ink and nigrosin
26
advantages of negative staining
no heat fixation, size ius more accurate
27
what type of stain is gram staining
differential stain
28
what is the purpose of the differential stain
it differentiates between two main classes of bacteria based on the composition of their cell walls.
29
what is the color of the thick layer of peptidoglycan for positive cells
purple color
30
what is the color of the thin layer of peptidoglycan for negative cells
violet stain
31
describe bacillus subtilis
rod, spore former, beneficial soil bacteria for healthy plant roots.
32
steps for gram staining
primary stain Mordant Decolorize Counterstain Beautiful results
33
what is the primary stain for gram staining
crystal violet
34
what is the mordant
gram's iodine
35
what is the decolorizing agent
Acetone/alcohol
36
what is the counterstain
safranin
37
what is special about acid fast bacilli
they have an inner membrane, thin peptidoglycan but no outer layer so doesn't retain crystal violet.
38
the purpose of acid fast carbon fuchsin method
used in diagnosis of leprosy or tuberculosis
39
Carbol fuchsin is
soluble in mycobacteria cell wall with detergent & heat
40
what is teh counterstain for acid-fast
methylene blue
41
acid fast organisms stain which color
red/pink
42
non acid fast organisms stain which color
blue with methylene blue
43
charcateristics of spores
metabolically inactive, highly resistant to heat, generate into new vegetative cells when conditions are favorable
44
spores do not retain with malachite green ---
the use of heat
45
vegetative cells counterstained
red with safranin
46
what does defined media mean
the exact cheical composition is known
47
what does complex media mean
exact chemical composition is unknown
48
example of complex
TS (Tryptic Soy)
49
what does selective media mean
can be complex or defined but supplemented with growth promoting orinhbitng additves
50
what does differential media mean
contains a dye or indicator used to distinguish from one organism to another
51
what does enriched media mean
contains added components to encourage the growth of fastidious organisms
52
example of selective
PEA Agar
53
Example of selective and differential
Mannitol Salt, Mac Conkey, EMB
54
example of enriched and differential
Blood Agar
55
does s.aureus or E.coli grow on non-selective media
Non-selective media
56
does S.aureus or E.coli grow on selective media
S.aureus grows well E.coli growth is inhibited
57
why is mannitol salt agar selectuve
because salt concentration inhibits most bacterial growth
58
why is mnanitol agar differential
some staphylococci can fermente the carbohydraet mannitol
59
what color is produced from fermenration of mannitol
red in 6-8-8.2
60
what does a yellow color on mannitol agar mean
below ph6.8
61
why is MacConkey Agar is selective
it contains crystal violet which inhibits cell redox
62
which bacteria grows in macconkey agar
negative bacteria
63
why is macconkey agar differential
bile salt nd neutral red pH allows differentiation of enteric bacteria
64
why is macconkey agar differential
bile salt nd neutral red pH allows differentiation of enteric bacteria
65
why is emb selective
partially inhibits growth of gm+ bacteria
66
why is emb differential
high lactic acid dyes surface into surface of colony
67
what organism grow on manntitol salt
s.epi, s.aureus
68
which organism grow in maconkey & EMB
samonella, E.coli, E.aerogenes
69
which organism grow in blood agar
E. aerogenes, E.faecaelis, A.faecelis
70
Blood agar is selective for
hemolytic strep, staph.spp
71
Mueller Hinton tellurite agar selective for
selective for corynebacteria
72
chocolate agar selective for
Neisseria spp, seperate for incubation, oxidase +
73
what does synder agar test determine
susceptibility to dental carries, measure acud oriduced by lactobacilli
74
what does lactobacilli contain
glucose fermenation + acid
75
what is HOMD
Human Oral Microbe Disease

micro bio lab exam 1 (10 decks)

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