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micro bio lab exam 1 > lab exam 1 > Flashcards

lab exam 1 Flashcards

1
Q

what goes in biohazard container

A

live container, liquid/solid culture,gloves

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2
Q

what goes in the general trash

A

paper towels, Kimwipes, paper and gloves

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3
Q

what goes in plastic pipettes

A

pl;astic loops, plastic needles

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4
Q

What is the objective of Aspetic Teechnique?

A

transfer of bacteria from one medium to another without introducing contaminants

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5
Q

What is the objective of subculturing

A

the transfer of microorganism from one medium to another for fresh growth

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6
Q

what is a pure culture

A

population of a single species

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7
Q

what is a mixed culture

A

population containing two or more species

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8
Q

streak plate is also called

A

streaking for singles

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9
Q

hwo do you streak plate

A

essentially a dilution, created by a loopful of culture over the surface of an agar plate

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10
Q

how do you spread plate

A

microorganism spread evenly throughout the plate

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11
Q

describe pseudomonas aeruginosa

A

ROD, can be pigmented, major cause of lung infection with cystic fibrous

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12
Q

describe staph, aureus

A

COCCUS, harmless skin bacteria to MRSA(pathogen)

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13
Q

Describe bacillus cereus

A

ROD, spore former, soil bacteria can cause food poisoning

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14
Q

which bacteria can be milky white to golden

A

S.aureus

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15
Q

purpose of bacterial smear

A

can be stained so as to better view the bacteria

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16
Q

what does a stain consist of

A

benzene ring, a chromophore, auxochrome

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17
Q

what does a chromophore do

A

imparts color

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18
Q

what does auxochrome

A

ionizes the chromogen

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19
Q

is a basic stain positively or negatively charged

A

positively charged

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20
Q

is a acid stain positively or negatively charged

A

negatively charges

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21
Q

examples of basic stains

A

methylene blue, carbon fuschin and crystal violet

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22
Q

correct exposure for methylene blue

A

1-2 min

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23
Q

correct exposure for crystal violet

A

20-60 secs

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24
Q

correct exposure for carbol fuchsin

A

15-30 sec

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25
Q

examples of acidic stain

A

india ink and nigrosin

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26
Q

advantages of negative staining

A

no heat fixation, size ius more accurate

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27
Q

what type of stain is gram staining

A

differential stain

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28
Q

what is the purpose of the differential stain

A

it differentiates between two main classes of bacteria based on the composition of their cell walls.

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29
Q

what is the color of the thick layer of peptidoglycan for positive cells

A

purple color

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30
Q

what is the color of the thin layer of peptidoglycan for negative cells

A

violet stain

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31
Q

describe bacillus subtilis

A

rod, spore former, beneficial soil bacteria for healthy plant roots.

32
Q

steps for gram staining

A

primary stain
Mordant
Decolorize
Counterstain
Beautiful results

33
Q

what is the primary stain for gram staining

A

crystal violet

34
Q

what is the mordant

A

gram’s iodine

35
Q

what is the decolorizing agent

A

Acetone/alcohol

36
Q

what is the counterstain

A

safranin

37
Q

what is special about acid fast bacilli

A

they have an inner membrane, thin peptidoglycan but no outer layer so doesn’t retain crystal violet.

38
Q

the purpose of acid fast carbon fuchsin method

A

used in diagnosis of leprosy or tuberculosis

39
Q

Carbol fuchsin is

A

soluble in mycobacteria cell wall with detergent & heat

40
Q

what is teh counterstain for acid-fast

A

methylene blue

41
Q

acid fast organisms stain which color

A

red/pink

42
Q

non acid fast organisms stain which color

A

blue with methylene blue

43
Q

charcateristics of spores

A

metabolically inactive, highly resistant to heat, generate into new vegetative cells when conditions are favorable

44
Q

spores do not retain with malachite green —

A

the use of heat

45
Q

vegetative cells counterstained

A

red with safranin

46
Q

what does defined media mean

A

the exact cheical composition is known

47
Q

what does complex media mean

A

exact chemical composition is unknown

48
Q

example of complex

A

TS (Tryptic Soy)

49
Q

what does selective media mean

A

can be complex or defined but supplemented with growth promoting orinhbitng additves

50
Q

what does differential media mean

A

contains a dye or indicator used to distinguish from one organism to another

51
Q

what does enriched media mean

A

contains added components to encourage the growth of fastidious organisms

52
Q

example of selective

A

PEA Agar

53
Q

Example of selective and differential

A

Mannitol Salt, Mac Conkey, EMB

54
Q

example of enriched and differential

A

Blood Agar

55
Q

does s.aureus or E.coli grow on non-selective media

A

Non-selective media

56
Q

does S.aureus or E.coli grow on selective media

A

S.aureus grows well
E.coli growth is inhibited

57
Q

why is mannitol salt agar selectuve

A

because salt concentration inhibits most bacterial growth

58
Q

why is mnanitol agar differential

A

some staphylococci can fermente the carbohydraet mannitol

59
Q

what color is produced from fermenration of mannitol

A

red in 6-8-8.2

60
Q

what does a yellow color on mannitol agar mean

A

below ph6.8

61
Q

why is MacConkey Agar is selective

A

it contains crystal violet which inhibits cell redox

62
Q

which bacteria grows in macconkey agar

A

negative bacteria

63
Q

why is macconkey agar differential

A

bile salt nd neutral red pH allows differentiation of enteric bacteria

64
Q

why is macconkey agar differential

A

bile salt nd neutral red pH allows differentiation of enteric bacteria

65
Q

why is emb selective

A

partially inhibits growth of gm+ bacteria

66
Q

why is emb differential

A

high lactic acid dyes surface into surface of colony

67
Q

what organism grow on manntitol salt

A

s.epi, s.aureus

68
Q

which organism grow in maconkey & EMB

A

samonella, E.coli, E.aerogenes

69
Q

which organism grow in blood agar

A

E. aerogenes, E.faecaelis, A.faecelis

70
Q

Blood agar is selective for

A

hemolytic strep, staph.spp

71
Q

Mueller Hinton tellurite agar selective for

A

selective for corynebacteria

72
Q

chocolate agar selective for

A

Neisseria spp, seperate for incubation, oxidase +

73
Q

what does synder agar test determine

A

susceptibility to dental carries, measure acud oriduced by lactobacilli

74
Q

what does lactobacilli contain

A

glucose fermenation + acid

75
Q

what is HOMD

A

Human Oral Microbe Disease

micro bio lab exam 1 (10 decks)

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