lab practical 1

Question 1

what shape is E. Coli

Answer 1

bacillus

Question 2

lyme disease and syphillus shape

Answer 2

spiral

Question 3

what is the purpose of aseptic techniques

Answer 3

prevents contamination of sample, handler, people around

Question 4

how to label plates

Answer 4

bottom of plates not the lid

Question 5

what is smear prep important to see

Answer 5

cell morphology, arrangmenet, internal structures

Question 6

what color are gram positive cells

Answer 6

purple

Question 7

what color are gram negative cells

Answer 7

pink

Question 8

which gram cell has the thickest peptidoglycan

Answer 8

gram positive

Question 9

primary gram stain

Answer 9

crystal violet, will stain both bacteria purple

Question 10

gram’s iodine

Answer 10

the mordant/fixing reagant

Question 11

ethyl alcohol

Answer 11

decolorizer in gram stain procedure, gram negative purple will be washed out

Question 12

safranin

Answer 12

gram counterstain, will color gram negative bacteria red/pink

Question 13

what is the cause of bacterial motility

Answer 13

chemotaxis

Question 14

what is true movement

Answer 14

chemotaxis, movement from point A to point B

Question 15

what is brownian motion

Answer 15

not true movement, just movement from being under the cover glass

Question 16

what is the purpose of pure culture techniques

Answer 16

to dilute bacteria concentration for just one colony

Question 17

what bacteria was used during pure culture

Answer 17

e. coli, s. aureus, p. aeruginosa

Question 18

what color is e. coli

Answer 18

white

Question 19

what color is p. aeruginosa

Answer 19

green

Question 20

what color is s. aureus

Answer 20

gold

Question 21

what is the purpose of the quadrant streak method

Answer 21

by the 4th streak there should hopefully be individual colonis

Question 22

what is the purpose of the pour plate technique

Answer 22

serial dilution

Question 23

what color are endospores

Answer 23

green

Question 24

what color are the vegetative/germinating cells

Answer 24

pink

Question 25

what is the acid fast bacteria?

Answer 25

mycobacterium smegmatus

Question 26

what color is the acid fast bacteria

Answer 26

m. smegmatus should be pink

Question 27

what color is the non acid fast bacteria?

Answer 27

blue/purple

Question 28

what is the non acid fast bacteria?

Answer 28

s. aureus

Question 29

what is present in the acid fast cell wall

Answer 29

mycolic acid

Question 30

what allows for dye penetration into the cell wall

Answer 30

phenol in fuschin

Question 31

can you see a bacteriophage without a microscope?

Answer 31

no

Question 32

how do we see bacteriophages?

Answer 32

using plaque essays

Question 33

what bacteria is used for plaque essays

Answer 33

e. coli B and T4

Question 34

confluent lawn is...

Answer 34

bacterial growth

Question 35

plaques are...

Answer 35

clearing of bacteria in a lawn due to lysis

Question 36

what bacteria is used during the motility lab

Answer 36

b. cereus, pneumoniae

Question 37

what shapes of bacteria do motility occur

Answer 37

rod and spiral shaped

Question 38

what motility techniques did we use

Answer 38

plate and tube stab, and hanging drop slide

Question 39

endospore stain procedure

Answer 39

malachite green for 10 min rinse with water 30 seconds counterstain with safranin 1 min

Question 40

acid fast stain procedure

Answer 40

fuschin 10 min wash with water decolorize with acid alcohol counterstain with methylene blue

Question 41

can you use the course focus knob on 40 and 100x?

Answer 41

no

Question 42

what kind of microscopy are we doing?

Answer 42

brightfield

Question 43

what materials did we use during the environmental bacteria experiment?

Answer 43

nutrient broth tubes and agar plates

Question 44

why do we spray down work station before starting lab?

Answer 44

to kill the vegetative cells and viruses

Question 45

what should you do before heat fixing the smear prep slide

Answer 45

air dry it

Question 46

what is the protein protective coat around the endospore?

Answer 46

the exosporium

Question 47

are brownian motion and chemotaxis the same thing?

Answer 47

no

Question 48

what part of the virus protects the nucleic acid

Answer 48

capsid

Question 49

plaque forming units are used to...

Answer 49

they can be counted to determine the number of viral particles effecting bacteria