Lab Practical 2 Flashcards
Fermentation of __________ in the PRS test is done by the enzyme ______
sucrose, sucrase
Fermentation of ________ in the PRL test is done by the enzyme _________
lactose, lactase
Phenol Red Result:
a color change indicates _______ has been metabolized
sugar
Phenol Red Result:
A yellow color indicates a _______ byproduct
acidic
Phenol Red Result:
a pink color indicates a _________ condition as a result of ________________
alkaline, peptone degradation
Phenol Red Result:
no color change or a slight orange color indicates _______
no reaction
Phenol Red Result:
a bubble in the Durham tube indicates ________
gas production
Urea Hydrolysis Result:
a ________ test results in a hot pink color which is the result of the pH change brought about by the resulting _______
Positive; ammonia
Urea Hydrolysis Result:
a pink color indicates the enzyme ______
urease
Urea Hydrolysis Result:
an orange color indicates __________
a negative reation
Catalase Test Result:
bubbles after the application of hydrogen peroxide indicates the presence of ________ and the organism is _________________
catalase; catalase positive
Citrate Test Result:
bacterial growth on the slant indicates a positive result for __________
citrate-permease
Citrate Test Result:
a blue color indicates the ________ byproducts of ______________ metabolism
alkaline; ammonium phosphatase
Starch Hydrolysis Result:
If the organism is positive for starch hydrolysis, a ______ zone will appear around the growth. A positive test indicates the presence of _________ and/or ________________.
clear; amylase and/or oligo-1, 6-glucosidase
Mannitol Salts Result:
If there is good growth on the plate, the bacteria is not inhibited by ________
7.5% NaCl
Mannitol Salts Result:
if there is no or poor growth on the plate, the bacteria is inhibited by _______
7.5% NaCl
Mannitol Salts Result:
if the growth and/or media turns yellow, the mannitol was hydrolyzed and ________________ is present
mannitol dehydrogenase
MacConkey Agar Result:
if there is no or poor growth on the plate, the bacteria is inhibited by __________ and ________
bile salts; crystal violet
MacConkey Agar Result:
if the growth and/or media are pink, ______ is produced from lactose fermentation by ____________
galactosidase
Eosin-Methylene Blue Agar Result:
little or no growth means __________ by one or both dyes
inhibition
Eosin-Methylene Blue Agar Result:
very dark colonies with or without a metallic sheen indicate _______ lactose fermentation and the presence of ___________
rapid; galactosidase
Eosin-Methylene Blue Agar Result:
colonies that are not dark purple to black indicate _______ lactose fermentation and/or _______ fermentation
slow; sucrose
Eosin-Methylene Blue Agar Result:
________ fermentation can be confirmed with a separate PRS test
sucrose
Methyl Red and Voges-Proskauer Tests Result:
a red color is a _________ indication for both tests
positive
