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Flashcards in Lab Quiz 2 Deck (53)
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1

(Prokaryotic) Enzyme

lower the activation energy of a reaction
- most are proteins with individual shapes determined by their unique amino acid sequence

enzyme

2

Activation Energy

The amount of energy needed to trigger a reaction.

enzyme

3

Active Site

- region where the enzyme binds that determines the catalytic effect of the enzyme.
- relative to the shape

enzyme

4

Cofactors

Aid in the binding process.

i.e. metallic ions (Fe3+, Mg2+, Ca2+, Mn2+), vitamins or other small molecules

enzyme

5

Coenzymes

Also aid in the binding process

i.e. metallic ions (Fe3+, Mg2+, Ca2+, Mn2+), vitamins or other small molecules

enzyme

6

Dye-coupled reactions

- The process of adding a dye into a reaction in order to observe the reaction between two things of interest.
- This technique is often used when using a spectrophotometer.

enzyme

7

Guaiacol

substrate

- colorless ==> brown (when oxidized)
- Used in the enzyme activity experiment, when oxidized it produces a brownish color.

enzyme

8

Derivative graph

The slope of the raw data and verses the derivatives of the lines at certain points on the graph.

enzyme

9

Denaturation

enzyme
When a protein loses its shape because of a change of external factors that differ from the native state.

10

Microplane Reader

enzyme
Detect differences between wells on a microtiter plate.

11

Spectrophotometry

enzyme
a method used to identify and quantify colored solutions based on their light absorbing properties

12

Karyokinesis

Mitosis
Nuclear division during mitosis.

13

Mitosis

mitosis
The process in which cells replicate and produce two identical daughter cells.

14

Cytokinesis

mitosis
Cytoplasmic divison, the last step in mitosis.

15

Chromosomes

mitosis
The carrier of genes found in the nucleus.

16

Sister Chromatids

mitosis
The duplicate parts of each chromosome.

17

Centrosomes

mitosis
Where the microtubule spindles meet. Spindles radiate from here.

18

Centromere

mitosis
Holds the sister chromatids together as part of the same chromosome.

19

Spindle

mitosis
Microtubules radiating from the centromere. Connect to sister chromatids at the kinetochore to pull them apart during mitosis.

20

Asters

mitosis
The "star-burst" structure made by the spindles and centrosome

21

Furrowing
(Cleavage Furrow)

mitosis
The indent or separation between the two daughter cells in the mitosis.

22

Cell Plate

mitosis
Found in plant cells, separates the daughter cells during the final stage of mitosis.

23

Cell Cycle

mitosis
The cell cycle is the events that take place leading up to and during cellular division.

24

Interphase

mitosis
the period of cellular growth prior to mitosis.

25

Prophase

mitosis
The nuclear envelope disappears, spindle begins to form.

26

Metaphase

mitosis
Spindle has arranged the chromosomes in the equator of the cell.

27

Anaphase

mitosis
Sister chromatids begin to separate and move to opposite poles of the cell.

28

Telophase

Mitosis
Chromosomes approach their individual poles, cleavage furrow begins to develop.

29

Blastula

Mitosis
A hollow sphere of cells surrounding intracellular fluid.

30

Fixative

mitosis
Used to preserve or stabilize specimens prior to microscopy.