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Flashcards in Lab Techniques Deck (3)
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1

ELISA

-enzyme-linked immunosorbent assays
-mix human blood cells with enzymes and antibodies
-quickest and easiest way to test presence of an antibody in a patient's serum
-often used to test for HIV

2

Process to test for presence of HIV using ELISA

(1) apply human serum to a plate that contains HIV antigens
*if anti-HIV antibodies are present in serum then they bind to the to the HIV antigens*
(2) plate is washed and secondary antibody specific for human antibodies is applied
(3) secondary antibody is covalently bound to an enzyme that produces a color change upon application of its substrate or to a molecule that will fluoresce

3

FISH

-fluorescence in situ hybridization
-used to search and find a specific DNA sequence on chromosomes
-use of PCR amplification to generate a supply of necessary probes
-Fluorescent probes that bind to only those parts of the chromosome with which they show a high degree of sequence similarity are used to tag sequences of DNA
-can identify a range of mutations including deletions, duplications, aneuploidy, and the presence of derivative chromosomes