Laboratory Safety (skipped)

Question 1

PASS
(fire extinguisher)

Answer 1

● P ull the pin
● A im at the base of fire
● S queeze handles
● S weep

Question 2

RACE

Answer 2

● R escue
● A larm
● C ontain
● E xtinguish

Question 3

MSDS

Answer 3

● M aterial
● S afety
● D ata
● S heet

Question 4

OSHA

Answer 4

● O ccupational
● S afety
● H ealth
● A dministration
● In US, issues regulation for
employee safety

Question 5

CAP

Answer 5

● C ollege of A merican P athologists

Question 6

CLSI

Answer 6

C linical and L aboratory S tandards
I nstitute

Question 7

CHP

Answer 7

C hemical H ygiene P lan

Question 8

EPA

Answer 8

E nvironmental P rotection Association

Question 9

NFPA

Answer 9

N ational F ire P rotection A ssociation

Question 10

ISO

Answer 10

I nternational O rganization for
S tandard

Question 11

CLIA

Answer 11

● C linical L aboratory I mprovement
A mendment
● In America, classifies if waived test or non-waived test

Question 12

either moderate complexity lab or high complexity

Answer 12

Non-waived test

Question 13

J oint C ommission I nternational

Answer 13

JCI

Question 14

What is the ISO for laboratory?

Answer 14

ISO 15189

Question 15

What agency of the government gives a permit to laboratories in the PH?

Answer 15

Answer: DOH branch is HFSRB (health
facilities and services regulation bureau)

Question 16

• “The application of safety and containment measures (practices, equipment, facilities) to minimize or prevent exposure from infectious agents to the person handling it in the laboratory, the laboratory, the building occupants, the community, and the environment.”

Answer 16

LABORATORY BIOSAFETY

Question 17

Which of the following statements regarding
Biosecurity is TRUE?
a. protection and prevention of unauthorized
possession, loss, theft, misuse, diversion, and
accidental or intentional release if biological
agents.
b. framework that describes the use of specific
practices, training and safety equipment.
c. refers to specially designed buildings to protect
the worker, community, and environment from an
accidental exposure or unintentional release of
infectious agents and toxins.
d. intentional release or dissemination of biological
agents.

Answer 17

A

Question 18

Anything that alters the structural component of the
laboratory

Answer 18

EXPOSURE CONTROL PLANS

Question 19

Basis for creation or revision of policies

Answer 19

RECORD KEEPING LOGBOOK

Question 20

RECORD KEEPING LOGBOOK

Must include the following:

Answer 20

1. Type and Brand of device involved in the incident
2. Location of the incident
3. Description of the incident

Question 21

An approach to infection control to which all human blood, tissue and most fluids are handled as if known to be infectious for the HIV, Hepatitis B, and other Blood Borne Viruses.

Answer 21

UNIVERSAL PRECAUTION

Question 22

Regulation that ensures that hazard information is properly communicated to employees.

Answer 22

HAZARD COMMUNICATION

Question 23

HAZARD COMMUNICATION

1. Ability to cause harm
2. Likelihood that a hazard will occur

Answer 23

1. Hazard
2. Risk

Question 24

Reduce or eliminate occupational exposure to hazardous chemicals.

Answer 24

CHEMICAL HYGIENE PLAN

Question 25

EMPLOYER’S RESPONSIBILITY | read

Answer 25

● Establish laboratory work methods and safety policies. ● Provide supervision and guidance to employees. ● Provide safety information, training, PPE, and medical surveillance to employees. ● Provide and maintain equipment and laboratory facilities that are free of recognized hazards and adequate for the tasks required. ● Maintain clean PPE used by more than one person ● Provision of Exposure Control Plan

Question 26

● Know and comply with the established laboratory safe work practices. ● Have a positive attitude toward supervisors, coworkers, facilities, and safety training. ● Be alert and give prompt notification of unsafe conditions or practices to the immediate supervisor and ensure that unsafe conditions and practices are corrected. ● Engage in the conduct of safe work practices and use of PPE.

Answer 26

EMPLOYEE’S RESPONSIBILITY

Question 27

1. Required to contain and expel noxious and hazardous fumes from chemical reagents . 1. It should be visually inspected for blockages by putting a piece of tissue paper at the good opening to indicate airflow direction

Answer 27

Fume Hood

Question 28

Remove particles that may be harmful to employees working with potentially infectious biologic specimens

Answer 28

Biosafety Cabinet

Question 29

# BSL 1. Ex: tuberculosis, francisella, SARS 2. Ex: Ebola, small pox 3. Organisms that do not cause disease in humans 4. Common bacteria Ex: staphylococcus

Answer 29

1. Level 3 1. 4 1. 1 1. 2

Question 30

# BIOSAFETY CABINET ● Clean bench ● Air re-circulates but it escapes making the user not well protected or vulnerable ● Filtrable agents: Virus ○ Size: < 0.3 um (not filtered by the biosafety cabinet)

Answer 30

CLASS I

Question 31

# BIOSAFETY CABINET ● Has both exhaust HEPA and supply HEPA ● Exhaust HEPA : the one that filters the air that comes out ● Supply HEPA : the one that filters the air that re-circulates ● Glass panel on class II biosafety cabinet only opens up to 8 inches

Answer 31

CLASS II

Question 32

# BIOSAFETY CABINET ● No air is recirculated ● Has a glove compartment and Negative air pressure

Answer 32

Class III

Question 33

Donning

Answer 33

Gown, Mask or respirator, goggles or face shield, gloves

Question 34

Doffing

Answer 34

Gloves Goggles or Face shield Gown Mask or aspirator

Question 35

Why are records kept for 30 years for those medical workers who worked in nuclear medicine or those exposed in radiation?

Answer 35

Because they are prone to developing cancer

Question 36

Equipment that emits a variety of wavelengths of electromagnetic radiation that must be protected against through engineered shielding or use of PPE

Answer 36

NON IONIZING

Question 37

NATIONAL FIRE PROTECTION ASSOCIATION (NFPA) HAZARD DIAMOND

Answer 37

● 4 - extreme hazard ● 3 - serious hazard ● 2 - moderate hazard ● 1 - slight hazard ● 0 - no or minimal hazard

Question 38

* Chemical reaction that involves the rapid oxidation of a combustible material or fuel with subsequent liberation of heat and light Triggering factors: ○ Fuel ○ Heat or ignition source ○ Oxygen (air)

Answer 38

FIRE

Question 39

# Class of Fire * Ordinary combustibles (Cloth, wood, paper, fabric) * Pressurized water, dry chemical and loaded steam * Don't use water on electrical fires or burining liquids

Answer 39

A

Question 40

# Class of Fire * Flammable * Liquids/gases or combustible petroleum liquids/products * Dry chemical, * CO2, Halon foam

Answer 40

B

Question 41

# Class of Fire * Energized Electrical Equipment * CO2, Dry chemical, and halon * Never use water. Dry chemical may damage electrical equipment. * CO, leave no residuem good choice for computers, analyzers

Answer 41

C

Question 42

# Class of Fire * Combustible/ Reactive metals/ flammable or combustible metal such as magnesium, sodium, and potassium * Metal X * Leave to professional fire fighters

Answer 42

D

Question 43

# Class of Fire * Detonation * Allowed to burn out and nearby material protected

Answer 43

E

Question 44

Component of a fire extinguisher that should be used for Class C fires (electrical equipment): A. Pressurized water and dry chemical B. Dry chemicals and carbon dioxide C. Carbon dioxide, halon, and dry chemical D. Metal X

Answer 44

C

Question 45

What is the best type of all-purpose fire extinguisher?

Answer 45

Answer : CO 2 foam

Question 46

* Have ground, polarity, and leakage checks and other periodic preventive maintenance performed on outlets and equipment. * The danger of electrocution in the laboratory is great due to the presence of multiple electrical equipment.

Answer 46

Electrical hazard

Question 47

Danger of fire, explosion, asphyxiation, or mechanical injuries

Answer 47

COMPRESSED GASES HAZARD

Question 48

* Liquid nitrogen * Danger of fire or explosion, asphyxiation, pressure buildup, embrittlement of materials, and tissue damage like thermal burns.

Answer 48

Cryogenic material hazard

Question 49

* Refers to equipment such as centrifuges, autoclaves and homogenizers, laboratory glassware, or glass pipets.

Answer 49

MECHANICAL HAZARD

Question 50

* Refers to the effect of repeated manipulation of instruments, containers, equipment, or repetitive strains such as tenpsynovitis, bursitis, and ganglion cysts. * Factors to consider: Position and posture; be mobile throughout the day. Applied force. Frequency of repetition. Design of hand tools. * Avoid: 1. Lifting heavy objects improperly. 1. Keeping the load close to the body using the muscles of the legs rather than the back. 1. Gradually increase force when pushing or pulling and avoid pounding actions with the extremities.

Answer 50

ERGONOMIC HAZARD

Question 51

Handling chemical spills

Answer 51

CHEMICAL HAZARD

Question 52

The best first aid is to flush the affected area with water for at least

Answer 52

15 minutes | Followed by appropriate medical attention.

Question 53

* Avoid running in the laboratory. * Watch wet floors * Avoid dangling jewelry * Tie back long hair * Seek help when lifting heavy objects.

Answer 53

PHYSICAL HAZARD

Question 54

Presented in: * Infectious material from patients Improperly processed blood products Inappropriately disposed waste products

Answer 54

BIOLOGICAL HAZARD

Question 55

* Encountered in the laboratory when procedures using radioisotopes are performed. * The amount of radiation in the laboratory setting is minute, but radiation effects are cumulative.

Answer 55

RADIOACTIVE HAZARD

Question 56

● Bunsen burners and organic chemicals ● Cause burns or dismemberment

Answer 56

FIRE HAZARD

Question 57

● Needles, lancet, and broken glass ● Cause cuts, punctures, or bloodborne pathogen expos

Answer 57

SHARP HAZARD

Question 58

TYPES OF NON-IONIZING RADIATION

Answer 58

pabasa nalang

Question 59

# WASTE DISPOSAL: COLOR CODING 1. Black 1. Green 1. Yellow 1. Yellow with black band 1. Orange

Answer 59

1. General waste 1. Wet waste 1. Infectious waste 1. Chemical and pharmaceutical waste 1. Radioactive waste

Question 60

BASIC FIRST AID PROCEDURES

Answer 60

Pabasa nalang