LEC EXAM #1 CHP. 3 Flashcards

(70 cards)

1
Q

How does bacteria move?

A

By changing the rotation of their flagella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is chemotaxis:

A
  • Causes spinning of flagella to move towards the attractant
  • Chemical attractants cause more runs
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Run vs. tumble:

A

RUN:

  • Flagella rotates in a counterclockwise direction
  • Flagella bundled

TUMBLE:

  • Flagella rotates in a clockwise direction
  • Not bundled
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Brownian motion vs directed movement:

A

Brownian motion:
the random movement of microscopic particles suspended in a gas or liquid due to collision of these particles in the fluid

Directed movement:
directed movement of cells up a conc. gradient

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

How does the presence of a capsule help bacteria survive?

A
  • Encapsulates them for more protection
  • Prevents WBC’s from phagocytosis
  • Hinders abx functions
  • Allows slime layer to adhere to a smooth surface, allowing it to stay for a longer amount of time
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is a pathogen?

A
  • Agent that causes diseases or illness to its host

- 4 types: bacteria, viruses, fungi, and parasites

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What types of molecules can move through the membrane freely (simple diffusion) or diffuse across a membrane?

A
  • Small, uncharged molecules
  • Hydrophobic molecules
  • O2, CO2, lipids, water
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What types of molecules require a carrier protein transporter for facilitative diffusion?

A
  • Large and charged molecules
  • Hydrophilic molecules
  • Glucose, carbs, amino acids, ions
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What types of molecules require active transport?

A

Glucose, ions, amino acids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Simple diffusion:

A

Molecules move DOWN their conc. gradient (high->low)

-Does not use energy to do this

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Active transport?

A
  • Pumps molecules against their conc. gradient using ATP (low-> high)
  • Uses ATP as energy to do this
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Facilitated diffusion:

A
  • Molecules move down their conc. gradient using the help of a carrier/transport protein (high->low)
  • Does not use energy to do this
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

How does osmosis work in prokaryotic cells? *

A

In prokaryotic cells, the cell wall provides some protection against changes in osmotic pressure, allowing it
to maintain its shape

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

The key morphological innovations of eukaryotic cells:

A
  • Has a nucleus that contains DNA
  • Has membrane bound organelles
  • Has a cytoskeleton that provides structure, keeps organelles in place, and helps with cellular transport
  • Has 80s proteins ribosomes
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

How do prokaryotic cells (7) differ from eukaryotic cells (6)?

A

Prokaryotic cells:

  • unicellular
  • smaller
  • more basic
  • came first
  • found in bacteria and archaea
  • no nucleus
  • no membrane bound organelles

Eukaryotic cells:

  • unicellular or multicellular
  • bigger
  • more complex
  • found in protists, plants fungi, and animals
  • has nucleus
  • has membrane bound organelles
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What do prokaryotic and eukaryotic cells have in common?

A

Both have:

  • DNA
  • Cell/plasma membrane
  • Cell wall (besides animal cells in eukaryotic cells)
  • Ribosomes
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

What is endomembrane trafficking?

A
  • Group of membrane organelles that produce and modify proteins and lipids
  • Starts in nuclear envelope goes to RER for protein synthesis or SER for lipid synthesis-> golgi apparatus-> packages it to plasma membrane or other parts of the cell
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

How does the RER differ from the smooth ER?

A

RER surrounds nuclear membrane and is bound by ribosomes-> makes proteins

Smooth ER makes lipids not proteins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Peroxisomes:

A

Membrane bound organelle that has lipid bilayer that breaks down peroxides to keep free radicals from forming

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Cytoskeleton:

A

Provides structure and support

-Network/highway for transportation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

3 cytoskeleton elements:

A
  • Microtubules
  • Microfilaments
  • Intermediate filaments
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Golgi apparatus:

A

Site where proteins are modified by placing sugar on proteins and ships them out to their final destination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Lysosome:

A

Garbage disposal of cell, breaks down the waste

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Nuclear lamina:

A

Intermediate filaments that support the nuclear membrane/gives it structure

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Centrosome:
Spindle fibers that segregate chromosomes in mitosis or meiosis
26
Ribosomes:
Synthesize RNA into protein in the RER
27
Microtubules:
- Highway of cell - Responsible for movement of cilia and flagella - Maintains shape of eukaryotic cells
28
How do we get things in and out of the nucleus?
DNA goes through nuclear pores that selectively allows you take things inside and outside the nucleus
29
Prokaryotic ribosomes:
70s
30
Eukaryotic ribosomes:
80s
31
60S + 40S=
80S ribosomal subunits
32
When vesicle pinches off from the RER, where does it go?
Cys side of golgi apparatus
33
Mitochondria: | 5
- Powerhouse of cell, makes ATP for cell - Comes from prokaryotic cells - Has 70S ribosomes, like bacteria - Has their own DNA - Creates cristae to increase surface volume
34
Plasma membrane: | 4
- Mobile, not static - Composed of lipid bayer and embedded proteins - Contains cholesterol that maintains membrane (IN EUKARYOTIC ONLY) - Contains glycoproteins and glycolipids that recognize other cells or pathogens
35
Prokaryotic and eukaryotic cells have the same:
PHOSPHOLIPID BILAYER
36
Eukaryotic plasma membrane performs:
Simple, facilitated, and active transport
37
3 processes that are SPECIFIC to eukaryotic cells:
Phagocytosis, pinocytosis, and receptor-mediated endocytosis
38
Extracellular matrix:
- Allows for external stress to be reduced on cells | - Acts as anchor for the cell
39
Microfilaments:
-Responsible for amoeboid movement, contractile ring formation, and pinching off ring
40
Intermediate filaments:
- Permeable - Composed of various proteins - Cell-to-cell contact
41
Subunit of microfilaments:
Actin
42
Subunit of microtubules:
Tubulin
43
Subunit of intermediate filaments:
Composed of various proteins such as keratin
44
Vesicular transport:
Membrane bound packets of protein made by the RER that are picked up by the golgi apparatus
45
Cilia:
Captures particules entering the airway and moves upwards out of airway
46
Dynein: | 2
- Protein in tubule that walks along microtubules using ATP | - Takes mitochondria in cell where ATP is needed
47
Cilia is made up of:
Microtubules
48
Flagella movement in prokaryotic cells?
Run and tumble
49
Flagella movement in eukaryotic cells?
Whipping
50
3 domains and which types of bacteria in each?
1. Archaea- prokaryotic 2. Bacteria- prokaryotic 3. Eukarya- eukaryotic
51
Endocytosis:
Selective of what we bring inside the cell
52
Phagocytosis:
Brings in food particles via ameboid movement
53
Pinocytosis:
Pulls in extracellular fluid for osmotic pressure
54
What happens to a cell in a hypertonic soln?
A soln that has a HIGHER solute conc. -> cell crenates
55
What happens to a cell in a hypotonic soln?
A soln that has a LOWER solute conc. -> cell lyses/swells
56
What happens to a cell in a isotonic soln?
No net movement of water particles-> overall conc. on both sides of cell membrane remains constant
57
Define tonicity:
Describes amount of particles in soln compared to the cell
58
Slime layer made of:
Glycocalyx and proteins
59
Osmosis:
Movement of water from HIGH-> LOW concentration
60
NAP:
DNA in prokaryotic cells interacts with NAPS (nucleoid associated proteins) which helps organize the DNA
61
Nucleoid:
Dense region of DNA in prokaryotic cell
62
3 variants of the cell wall:
- Gram negative - Gram positive - Acid-fast
63
Gram negative: | inner to outer
- Inner membrane - Periplasmic space - Thin peptidoglycan cell wall - Lipoprotein - Outer membrane - LPS endotoxin - Harder to treat with abx
64
Acid fast:
Absorbed by mycolic acid which acts as a waxy coat
65
Gram positive: | inner to outer
- Inner plasma membrane - Thick peptidoglycan cell wall - Teichoic acid: gives rigidity and allows to bind to host cells glycoproteins - Acid fast stain on outside - Easier to treat with abx
66
Gram negative structure:
- 5 proteins horizontally and 4 proteins vertically | - Peptidoglycan crosslink
67
What membrane bound organelles do eukaryotes have?
Nucleus, mitochondria, golgi, ER
68
How does osmosis work in cells that do not have a cell wall?
In cells that lack a cell wall, changes in osmotic pressure can lead to crenation in hypertonic environments or cell lysis in hypotonic environment
69
ALL gram negative have:
LPS that sticks out on outer membrane
70
Gram positive structure:
- 4 proteins vertically only - Direct link from tetra to tetra - No peptidoglycan cross link