Lecture 14: Genomics

Question 1

genome

Answer 1

entire complement of genetic information

Question 2

genomics

Answer 2

discipline of mapping, sequencing, analyzing & comparing genomes

Question 3

what was the first genome sequenced

Answer 3

RNA virus MS2

Question 4

sequencing

Answer 4

determining precise order of nucleotides in DNA/RNA sequence

Question 5

generation

Answer 5

successive major changes in sequencing technology

Question 6

sanger method is an example of which generation sequencing

Answer 6

1st

Question 7

which generation sequencing is used in labs?

Answer 7

2nd

Question 8

sanger method

Answer 8

chain termination

Question 9

what prevents further extension of DNA in the sanger method

Answer 9

analogs

Question 10

pyrosequencing

Answer 10

DNA is broken into small fragments & can handle only short stretches of DNA

Question 11

example of 3rd generation sequencing method

Answer 11

minion

Question 12

genome assembly

Answer 12

puts fragments in the correct order & eliminates any overlaps

Question 13

functional ORF

Answer 13

encodes a protein and can be identified by a computer

Question 14

annotation

Answer 14

converts raw sequence data into genes present in the genome

Question 15

annotation is ( ) in genomics

Answer 15

bottleneck

Question 16

bioinformatics

Answer 16

applies technology tools to DNA and protein sequences for comparative purposes

Question 17

hypothetical proteins

Answer 17

uncharacterized ORFs

Question 18

noncoding RNA

Answer 18

RNA that does not code for proteins

Question 19

as genome size increases, gene content ( )

Answer 19

increases

Question 20

what is the minimum number of genes for a viable cell

Answer 20

250-300

Question 21

what type of genes are most abundant?

Answer 21

metabolic

Question 22

number of genes that can be identified in a given genome is

Answer 22

70% or less of total ORFs detected

Question 23

Archaea devote a ( ) percentage of their genome to energy than bacteria

Answer 23

higher

Question 24

Archaea contain ( ) genes for carbohydrate metabolism functions than bacteria

Answer 24

fewer