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BMSC 210 > Lecture 7: Translation & Protein Processing > Flashcards

Lecture 7: Translation & Protein Processing Flashcards

1
Q

what type of RNA is translated?

A

mRNA

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2
Q

what do sRNA base pair with?

A

mRNA

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3
Q

what is sRNA used for?

A

silence genes & activate/repress translation

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4
Q

what is the intergenic region?

A

area between genes that has a low degree of complementary

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5
Q

what does sRNA do when there is a change in secondary structure?

A

inhibit or stimulate translation

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6
Q

what does sRNA do when there is a change in mRNA stability?

A

degrades & protects RNA

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7
Q

what is translation?

A

production of a protein

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8
Q

tertiary structure

A

folded proteins

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9
Q

Quaternary structure

A

two or more polypeptides

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10
Q

what type of RNA is an anticodon?

A

tRNA

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11
Q

what is the wobble position?

A

3rd position of codon that can change the 3rd letter

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12
Q

what is codon bias?

A

not all bacteria use the same set of codons

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13
Q

what is the A-site

A

acceptor where tRNA arrives & binds to it

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14
Q

what is the P-site

A

where the peptide grows

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15
Q

what is the E-site

A

where tRNA is ejected

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16
Q

what is the 30S?

A

smaller subunit that binds to mRNA

17
Q

what is the 50S?

A

larger subunit that catalyzes the sites

18
Q

what do polysomes mean?

A

multiple ribosomes produced for 1 mRNA

19
Q

are chaperones actively used during translation?

A

yes

20
Q

what 4 things do chaperones help with?

A
  1. energy dependent process
  2. folding & refolding
  3. add co-factors
  4. help secretion
21
Q

how is enzymatic activity changed?

A

post translational modifcation

22
Q

what is sequestration?

A

the binding partner

23
Q

what does the translocase do?

A

translocate protein from cytoplasm to outside (gram pos) or periplasm (gram neg)

24
Q

what does the SEC system do?

A

transports non-folded protein & export it outside cell

25
Q

what does the TAT system do?

A

transports folded proteins across membrane

26
Q

how long is the signal sequence?

A

15-20 amino acids

27
Q

what happens if there is no signal sequence?

A

there would be no movement

28
Q

what transporter does Type I use?

A

ABC transporter

29
Q

what transporter does Type II use?

A

SEC & TAT systems

30
Q

what transporters does Type V use?

A

autotransporter & SEC/TAT

31
Q

what does Type III do?

A

inject proteins into eukaryotic cells

32
Q

what does Type IV do?

A

transfer DNA

33
Q

what does Type VI do?

A

bacteria kills other bacteria

BMSC 210 (16 decks)

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