lecture 20 redone

Question 1

ON mechanism cAMP production
cAMP production is via the enzyme

Answer 1

adenylyl cyclase

Question 2

there are 2 different types of adenylyl cyclase

Answer 2

1. in the plasma membrane
2. soluble enzyme exists in the cytosol

Question 3

what domains to type 1 have

Answer 3

2 TMDs and 2 catalytic domains (C1 and C2)

Question 4

in the off state the catalytic domains are

Answer 4

separated

Question 5

when activated the catalytic domains

Answer 5

are together

Question 6

when activated the ATP can be converted into

Answer 6

cAMP

Question 7

type 2 is activated by

Answer 7

increased HCO and Ca

Question 8

forskolin from plant alkaloid

Answer 8

actiavtes the enzyme

Question 9

OFF mechanism cAMP breakdown

Answer 9

phosphodiesterase (PDEs)

Question 10

cilastazol is a

Answer 10

PDE3 inhibitor for peripheral vascular disease (cAMP vasodilation to improve blood flow)

Question 11

CFTR agonist example

Answer 11

adenosine

Question 12

AKAPS bind to

Answer 12

PKA

Question 13

2 types of PKA

Answer 13

type II can bind to AKAPs it has a docking domain which it can bind to, AKAPs bring the sibstrate close to the catalytic subunits in type II

Question 14

HT31 peptide disrupts

Answer 14

PKA-AKAP interaction

Question 15

ezrin (AKAP) targets PKA to CFTR this requires a

Answer 15

scaffold protein (NHERF1)

Question 16

phospholamban binds to serca and requlates it negatively

Question 17

overexpression of NHERF1 rescued

Answer 17

f508 CFTR to the membrane in CF