Lecture 4 Flashcards

1
Q

Rational protein design is the same as directed evolution

A

False. Irrational protein design is the same as directed evolution

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2
Q

The two types of irrational protein design are error prone PCR and DNA shuffling

A

True

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3
Q

The normal function of Taq polymerase is adding nucleotides to the growing strand and proof reading

A

True

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4
Q

Having no ddATP in PCR has no effect.

A

False. It causes in incorrect base to be put in instead

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5
Q

Error prone PCR is also known as PCR with high fidelity

A

False. It is known as PCR with low fidelity

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6
Q

Homologous genes are derived from the same ancestors

A

True

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7
Q

Dnase has high specificity

A

False. It has low specificity

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8
Q

Restriction enzymes are specific

A

True

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9
Q

Overhanging primers are a starting point for PCR

A

True

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10
Q

RFLP uses homologous genes

A

True

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11
Q

Primerless PCR is a component of gene shuffling

A

True

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12
Q

DNase I produces smaller fragments than restriction enzymes

A

False. Restriction enzymes creates smaller fragments than DNase I

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