Lecture 6 Flashcards
(25 cards)
What is the definition of mutation
Heritable change in the nucleotide in the DNA cause alteration in genetic information
What is the importance of mutation
Cause changes and adaptation
What happen when GTPase mutated
GTP will not be able to hydrolysis GTP to GDP which will make RAS always activated and cause cancer
What cause skin cancer
UV radiation
What cause lung cancer
Smoking
What are the two strategies of anti cancer to kill tumor cell
Radiotherapy or chemotherapy
What are the endogenous causes of DNA damage
DNA replication error
Free radical
Base deamination
What is the exogenous cause of cancer
Radiation and smoking
When there is Damage in the DNA base which repaire mechanism is used
Base excision repair
What cause APsite formation in BER
DNA glycosylase
Where does AP endonuclease cleaves DNA
At the AP site
Which enzym3 come with the repaired piece
DNA polymerase
Which enzyme that joins the DNA
DNA ligase
Xeroderma pigmentosum (XP) is caused by ?
Lack of DNA repair from UV radiation and mutation in XP gene
XP can lead to ?
Skin cancer
IN Nucleotide excision repair ( NER) the damaged base is recognized by ?
complex containing XPA and XPC and DNA polymerase
In NER the DNA is unwind by
XPB , XPD
In NER the DNA lesion is cut by ?
XPF , XPG
Genome is replicated in which phase
S phase
In mismatch repair (MMR) what are the recognition proteins
MSH2 , MSH6
In MMR the DNA is cut by
Sliding clamp
What may cause double strands break
Ionizing radiation
What are the types of ionizing radiation
X-ray , gamma ray
Ionizing radiation have ……. Wavelength……….. energy
Short , high
