Lecture 8

Question 1

picornaviruses

Answer 1

T=1 
naked
enteroviruses,
poliovirus, coxsackievirus, echoviruses
rhinovirus
hepatovirus, cardiovirus, aphthovirus

Question 2

poliovirus, coxsackievirus, echovirus

Answer 2

hardy - at pH 3-5 for 1-3 hours
fecal-oral
inactive with chlorine, UV, formaldehyde, heat

Question 3

rhinovirus

Answer 3

sensitive to acidic pH

Question 4

picornavirus human affecting

Answer 4

human rhinovirus
hepatitis A virus
poliovirus

Question 5

picornavirus genome

Answer 5

linear +ssRNA
short poly A tail on 3’ end
viral protein VPg on 5’ end
4 capsid proteins

Question 6

poliovirus entry

Answer 6

VP1 capsid protein serves as ligand
N-termini push into plasma membrane and make a channel
VPg and RNA enter cytoplasm

Question 7

uncoated genome

Answer 7

outcompetes cellular mRNA

cellular mRNA does not find ribosomes

Question 8

poliovirus

Answer 8

many (~90%) asymptomatic case
fecal-oral but not enteric
infect skeletal muscle then innervating neurons

Question 9

coronavirus

Answer 9

helical capsid
N protein is nucleocapsid
nucleocapsid forms “coil”
+ssRNA genome

Question 10

coronavirus human affecting

Answer 10

SARS

HKU1

Question 11

SARS

Answer 11

severe acute respiratory syndrome (hypoxemia)

Question 12

HKU1

Answer 12

upper respiratory infections

Question 13

coronavirus genome

Answer 13

largest known RNA genome

-ssRNA is template for genomic RNA synthesis

Question 14

coronavirus entry

Answer 14

many types of receptors

ACE-2