Lecture 9 Flashcards Preview

BMS242 - Cell and Molecular > Lecture 9 > Flashcards

Flashcards in Lecture 9 Deck (9)
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1

what is the SEC pathway

ER - Golgi - PM/endosome/lysosome

2

what genetic organisms are commonly used to study membrane trafficking

yeast
mice
zebrafish
C.elegans

3

what are advantages of using yeast

easily controlled
entire genome known
cheap and easy to grow
limited gene diversity
fundamental pathways conserved

4

what are disadvantages of yeast

limited cell-cell contact
small - high resolution imaging needed
cell wall

5

how many genes were discovered in relation to SEC pathway

23
class A - accumulation in cytosol
class B - accumulation in RER
class C - accumulation in ER-Golgi vesicles
class D - accumulation in Golgi
class E - accumulation in secretory vesicles

6

why werent all genes discovered

only identified temperature sensitive mutants
only did secretion to PM
any reduntantly functioning genes would not be identified

7

why is endocytosis important

retrieval of molecules
down regulation if signals
remodelling cell surface

8

how many genes were identified in VPS

>60
CPY was followed as normally excreted - gycosylated and proteolytically cleaved at different stages so can be followed

9

MVB

multi vesicular body