LESSON 1 LAB SAFETY Flashcards

1
Q
  • Authorized to conduct on on-site inspections to
    determine whether an employer is complying
    with the mandatory standards (Bishop, 2013)
  • Organization that encompasses all workplace
    area (not just laboratories)
  • Philippines: Department of Labor and
    Employment and Department of Health
  • Most of their policies are being used by the
    agencies here in the Philippines
A

OCCUPATIONAL SAFETY AND HEALTH
ADMINISTRATION (OSHA)

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2
Q

• Bloodborne Pathogen Standard
• Formaldehyde Standard
• Laboratory Standard
• Hazard Communication Standard
• Respiratory Protection Standard Air
• Contaminants Standard
• Personal Protective Equipment Standar

A

Standards that regulate safety in the laboratory:

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3
Q
  • provides excellent general laboratory safety and
    infection control guidelines
  • more specific in procedures
  • step by step laboratory procedures
A

CLINICAL LABORATORY STANDARDS
INSTITUTE (CLSI)

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4
Q
  • Standard System for the Identification of the Fire
    Hazards of Materials, (NFPA 704)
A

NATIONAL FIRE PROTECTION
ASSOCIATION (NFPA)

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5
Q

Health Hazard

A

blue diamond

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6
Q

Fire hazard

A

red diamond

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7
Q

specific hazard

A

white diamond

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8
Q

reactivity

A

yellow diamond

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9
Q
  • All Patients are considered possible carriers of
    bloodborne pathogens (HIV and HBV)
  • Wearing gloves when collecting/ handling blood
    and body fluids visibly contaminated with blood
  • Wearing face shields when in danger of splashing
    blood and body fluids contaminated with blood - Doesn’t include urine and fluids not visibly
    contaminated with blood
  • First set of guidelines on how to approach
    patients and specimens
A

Universal Precautions (1987)

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10
Q
  • Considers all body fluids and moist body
    substances to be potentially infectious
  • Does not recommend handwashing after
    removal of gloves
A

Body Substance Isolation Guidelines (1987)

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11
Q
  • Combines aspects of Universal Precautions and
    Body Substance Isolation Guidelines
  • Way of handling the patients
A

Standard Precautions (1996)

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12
Q
  • Accreditation of Clinical Laboratories (US)
A

THE JOINT COMMISSION

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13
Q
  • Development of policies regarding laboratory
    safety
  • Accreditation of Clinical Laboratories
  • International Organization for Standardization
    (ISO) also inspects clinical laboratories in the
    Philippines
A

DEPARTMENT OF HEALTH (PHILIPPINES)

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14
Q

Guidelines in laboratory setting

STANDARD PRECAUTIONS (CDC, 1996)

A

Handwashing,
gloves,

Masks, Goggles, and Face Shields

➢ Laboratory Gown

➢ Occupational Health ➢ Patient Care Equipment
➢ Environmental Control
➢ Linen
➢ Patient Placement

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15
Q

o Before patient contact
o When gloves are removed
o Before leaving the work area
o At any time when they have been
knowingly contaminated
o Before going to designated break areas
o Before and after using bathroom
facilities

A

➢ Handwashing

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16
Q

o worn to protect the health care worker’s
hands from contamination by patient
body substances and to protect the
patient from possible microorganisms
on the health care worker’s hands.
o NOT a substitute for hand washing
o Latex allergy in patients

A

Gloves

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17
Q

o Masks are worn to protect against
inhalation of droplets containing
microorganisms from infective patients.
o Masks and goggles are worn to protect
the mucous membranes of the mouth,
nose, and eyes from splashing of body
substances
o Face shields also protect the mucous
membranes from splashes.

A

➢ Masks, Goggles, and Face Shields

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18
Q

o worn to protect the clothing and skin of
health care workers from contamination
by patient body substances and to
prevent the transfer of microorganisms
out of patient rooms.
o tie in the back at the neck and the waist
and have tightfitting cuffs

A

Laboratory Gown

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19
Q

o Never recap used needles using both
hands
o Place used disposable syringes and
needles, scalpel blades, and other sharp
items in appropriate puncture resistant
containers

A

Occupational Health

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20
Q
  • requires all employers to have a written
    Bloodborne Pathogen Exposure Control Plan and
    to provide necessary protection, free of charge
    for employees
A

OSHA (Occupational Safety and Health
Administration)

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21
Q

Components of the OSHA Bloodborne Pathogen
Standard

o Providing sharps disposal containers and
needles with safety devices.
o Requiring discarding of needles with the
safety device activated and the holder
attached
o Labeling all biohazardous materials and
containers

A

Engineering Controls

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22
Q

Components of the OSHA Bloodborne Pathogen
Standard o Requiring all employees to practice
Standard Precautions.
o Prohibiting eating, drinking, smoking,
and applying cosmetics in the work area.
o Establishing a daily work surface
disinfection protocol.

A

Work Practice Controls

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23
Q

Components of the OSHA Bloodborne Pathogen
Standard o Providing laboratory coats, gowns, face
shields, and gloves to employees and
laundry facilities for non-disposable
protective clothing

A

Personal Protective Equipment

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24
Q

Components of the OSHA Bloodborne Pathogen
Standard

o Providing immunization for the hepatitis
B virus free of charge.
o Providing medical follow-up to
employees who have been accidentally
exposed to bloodborne pathogens.

A

Medical

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25
Components of the OSHA Bloodborne Pathogen Standard o Documenting annual training of employees in safety standards. o Documenting evaluations and implementation of safer needle devices. o Involving employees in the selection and evaluation of new devices and maintaining a list of those employees and the evaluations. o Maintaining a sharps injury log including the type and brand of safety device, location, and description of the incident, and confidential employee follow-up.
Documentation
26
- Any accidental exposure to blood through needlestick, mucous membranes, or nonintact skin must be reported to a supervisor and a confidential medical examination must be started immediately - Evaluation of the incident must begin immediately to ensure appropriate postexposure prophylaxis (PEP) is initiated within 24 hours.
Postexposure Prophylaxis
27
Color Coding of Waste Containers (from DOH) Non-infectious dry waste
black
28
Color Coding of Waste Containers (from DOH) Non-infectious wet waste (kitchen, dietary, etc.)
Green
29
Color Coding of Waste Containers (from DOH) Infectious and pathological waste
Yellow
30
Color Coding of Waste Containers (from DOH) Chemical waste including those with heavy metals
Yellow with black band
31
Color Coding of Waste Containers (from DOH) Radioactive waste
Orange
32
Color Coding of Waste Containers (from DOH) Sharps (needles and glasswares) and pressurized containers
Red
33
- describes hazards, safe handling, storage, and disposal of hazardous chemicals.
Safety Data Sheet (SDS)
34
Source- Infectious agents POSSIBLE INJURY- Bacterial, fungal, viral, or parasitic infections
biological
35
Needles, lancets, and broken glass Cuts, punctures, or bloodborne pathogen exposure
Sharp
36
Preservatives and reagents Exposure to poisonous, caustic, or carcinogenic agents
chemical
37
Equipment and radioisotopes Damage to a fetus or generalized overexposure to radiation
Radioactive
38
Ungrounded or wet equipment and frayed cords Burns or shock
Electrical
39
Open flames and organic chemicals Burns or dismemberment
Fire/Explosive
40
Wet floors, heavy boxes, and patients Falls, sprains, or strains
Physical
41
- refers to biological substances that pose a threat to the health of living organisms, primarily that of humans. - contain any of the four microorganism - virus, bacteria, fungi, parasite - laboratory specimens
BIOHAZARDS
42
o Consist of bacteria, fungi, parasites, and viruses.
Infectious Agent
43
▪ Early detection and treatment of infectious agents.
Breaking the Chain:
44
o A place where the infectious agent can live and possibly multiply. o Ideal ______: Humans and Animals (they do not exhibit symptoms) o Equipment and other soiled objects (fomites)
Reservoir
45
▪ Disinfecting the work area kills the infectious agent and eliminates the reservoir.
Breaking the Chain
46
o A way to exit the reservoir to continue the chain of infection o Nose, mouth, and mucous membranes and in blood or other body fluids
Portal of Exit
47
▪ Disposing of needles and lancets in sealed sharps containers ▪ Disposing of contaminated materials in biohazard containers ▪ Keeping tubes and sample containers sealed.
Breaking the Chain:
48
unprotected host touches or is touched by the reservoir
Direct contact
49
the host inhales material from the reservoir such as aerosol droplets from an infected person (too large to remain suspended in the air
Droplet
50
inhalation of dried aerosol nuclei circulating on air currents or attached to dust particle (usually for viruses; too small to remain suspended in the air)
Airborne
51
ingestion of contaminated food or water
Vehicle
52
parasites such as malaria transmitted by a mosquito bite (transportation of microorganism)
Vector
53
the means of transmission can be an accidental needlestick.
phlebotomist
54
▪ Hand washing ▪ Standard Precautions ▪ Transmission-based precautions
Breaking the Chain:
55
o Means to enter the reservoir o Nose, mouth, mucous membranes, and open wounds.
Portal of Entry
56
▪ Disinfection and sterilization ▪ Strict adherence to Standard precautions and transmission-based precautions
Breaking the Chain
57
o Can be another patient or the health health-care provider. o Ideal ________: Patients (they are immunocompromised)
Susceptible Host
58
▪ Observation of special precautions when working in the nursery and in isolation rooms designated for protection of susceptible patients. ▪ Immunizations ▪ Maintenance of a healthy lifestyle is very important for the health -care worker.
Breaking the Chain
59
- an infection acquired by a patient during a hospital stay. - Reasons: Unsanitary conditions and immunocompromised patients
Nosocomial/Healthcare Acquired Infections
60
- refers to an infection acquired by a patient as the result of a health care procedure that may or may not require a hospital stay.
Health care acquired infection (HAI)
61
- Including needles, lancets, and broken glassware - Serious biological hazard for possible exposure to bloodborne pathogens caused by accidental puncture - When handling needles, NEVER manually recap one-handed - Needle safety devices must be activated before disposing of the entire blood collection assembly.
SHARPS HAZARDS
62
- Puncture-resistant, leak-proof containers labeled with the biohazard symbol. - Containers should be located in close proximity to the phlebotomist’s work area.
Disposal of Sharps
63
- When skin or eye contact occurs, the best first aid is to: o Flush the area immediately with water for at least 15 minutes and then seek medical attention o Know the location of and how to use the emergency shower and eyewash station in the laboratory o Safety Showers
CHEMICAL HAZARDS
64
30-50 gal/minute at 20-50 psi
Safety Showers
65
Disinfect the spill site using approved disinfectant
10% bleach,
66
o Works like an exhaust fan o For chemical reagents only
Chemical Fume Hoods
67
o For opening specimens o Any airborne contaminants will be pushed down to filter out microorganism
Biosafety Cabinets
68
- Encountered in the clinical laboratory when procedures using radioisotopes, such as radioimmunoassay, are performed. - Exposure to radiation during pregnancy presents a danger to the fetus, and personnel who are or who think they may be pregnant should avoid areas with this symbol.
RADIOACTIVE HAZARDS
69
- Electrical cords should be checked regularly for fraying and replaced - All sockets should be checked for electrical grounding and leakage at least annually. - No extension cords should be used in the laboratory
ELECTRICAL HAZARDS
70
FIRE TYPE: _____ COMPOSITION OF FIRE: Wood, paper, or clothing TYPE OF FIRE EXTINGUISHER: _______ EXTINGUISHING MATERIAL: Water
Class A
71
FIRE TYPE:_______ COMPOSITION OF FIRE: Flammable organic chemicals TYPE OF FIRE EXTINGUISHER: _______ EXTINGUISHING MATERIAL: Dry chemicals, carbon dioxide, foam, or Halon
Class B
72
FIRE TYPE:_______ COMPOSITION OF FIRE: Electrical TYPE OF FIRE EXTINGUISHER: ______ EXTINGUISHING MATERIAL: Dry chemicals, carbon dioxide, or Halon
Class C
73
FIRE TYPE:______ COMPOSITION OF FIRE: Combustible metals TYPE OF FIRE EXTINGUISHER: _______ EXTINGUISHING MATERIAL: Sand or dry powder Dry chemicals
Class D None Class ABC
74
FIRE TYPE: ______ COMPOSITION OF FIRE: Grease, oils, fats TYPE OF FIRE EXTINGUISHER: _______ EXTINGUISHING MATERIAL: Liquid designed to prevent splashing and cool the fire
Class K
75
PASS
Pull, Aim, Squeeze, sweep
76
RACE
Rescue, Alarm, Contain, extinguish/evacuate
77
• Avoid running in rooms and hallways. • Be alert for wet floors. • Bend the knees when lifting heavy objects or patients. • Keep long hair tied back and remove dangling jewelry to avoid contact with equipment and patients. • Wear comfortable, closed toe shoes with nonskid soles that provide maximum su
PHYSICAL HAZARDS
78
Cleaning Reagents
1:10 dilution of chlorine bleach 70% Ethanol solution • 5% Phenolic Solution (Lysol)
79
- Safety carriers should always be used to transport glass bottles of acids, alkalis, or organic solvents in volumes larger than 500 mL, - Approved safety cans should be used for storing, dispensing, or disposing of flammables in volumes greater than 1 quart - Steel safety cabinets with self-closing doors or explosion proof refrigerators
Chemical Storage
80
- Manufacturers of laboratory chemicals - All in-house prepared reagents and solutions should be labeled in a standard manner and include the o chemical identity o concentration o hazard warning o special handling o storage conditions o date prepared o expiration date (if applicable) o preparer’s initials.
Signage and Labelling