lesson 9 Flashcards
(27 cards)
% of laboratory errors that occur prior to analysis
46%-68%
what are the routine handling
- MIXING TUBES BY INVERSION
- TRANSPORTING SPECIMENS
rupture of RBC
hemolyze
Special Handing
- BODY TEMPERATURE
- CHILLED SPECIMEN
- LIGHT-SENSITIVE SPECIMEN
can hold temperature for 15 minutes
portable heat blocks
used for specimens that can withstand a temperature slightly higher than 37C
HEEL WARMER
examples in body temperature
- COLD AGGLUTININ
- CYROFIBRINOGEN
- CYROGLOBULINS
slows metabolic process
CHILLING
examples of chilled specimens
Adrenocorticotropic hormone (ACTH), acetone, angiotensin-converting enzyme (ACE), ammonia, catecholamines, free fatty acids, gastrin, glucagon, homocysteine, lactic acid, parathyroid hormone (PTH), ph/blood gas (if indicated), pyruvate, and renin
There are cases when exposure to light can affect the result of a specimen
LIGHT SENSITIVE SPECIMEN
examples of light sensitive specimens
Bilirubin, carotene, red cell folate, serum folate, Vitamin B, Vitamin B, Vitamin B, Vitamin C, urine porphyrins, and urine porphobilinogen
routine blood specimen is expected to reach the laboratory within __
45 minutes
centrifugation needed by specimen should be done in _____
1 hour
delivery time limit exemption examples
STAT or EMERGENCY
Blood smear from EDTA specimen
1 hour from collection
EDTA specimen for CBC
analyzed within 6 hours
stable 24 hours from collection
EDTA specimen for erythrocyte sedimentation rate (ESR) determination
tested 4 hours (room temperature) and 12 hours (refrigerated).
EDTA specimen for reticulocyte counts
stable for 6 hours (room temperature) 72 hours if refrigerated.
Glucose test drawn in sodium fluoride tubes
-stable for 24 hours (roomtemperature)
-but stable for up to 48 hours when refrigerated at 2°C to 8°C
apparatus that is used to separate cells, plasma, or serum of blood specimens.
CENTRIFUGE
is achieved by spinning the blood tubes inside the vessel at a high speed such that the centrifugal force will cause the separation of the specimens.
CENTRIFUGATION
tubes of the same size and volume should be
placed opposite to one another
what specimen needs to be completely clotted prior to centrifugation
SERUM SPECIMEN
Prothrombin time (PT) & (PTT)
(PT) - stable for 24 hours
(PTT) - should be analyzed within 4 hours from collection.