Lippincott - Protein Synthesis Flashcards Preview

[OS 201] 3rd Exam > Lippincott - Protein Synthesis > Flashcards

Flashcards in Lippincott - Protein Synthesis Deck (56)
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1

direction of writing of codon

5'-->3'

2

number of base combinations

64

3

number of codons coding for amino acids

61

4

stop codons

UAA, UAG, UGA

5

start codon

AUG

6

protein coded for by start codon

methionine

7

characteristics of genetic code [4]

[1] specificity / unambiguity
[2] universality
[3] redundance / degeneracy
[4] nonoverlapping

8

specificity of genetic code

a particular codon codes for only one amino acid

9

universality of genetic code

genetic code found in all organisms

10

redundance / degeneracy of genetic code

a single amino acid has more than 1 coding codon

11

nonverlapping / commaless

code is read from a fixed starting point as a continuous sequence of bases

12

consequences of altering nucleotide sequence [6]

[1] silent mutation
[2] missense
[3] nonsense
[4] triple repeat expansion
[5] splice site mutation
[6] frameshift

13

silent mutation

single nucleotide mutation does not change the amino acid sequence

14

missense

single nucleotide mutation changes amino acid sequence

15

nonsense

single nucleotide mutation produces stop codon

16

result of nonsense mutation

truncated protein

17

triple repeat expansion

amplification of a codon resulting in abnormally high amounts of a single amino acid

18

splice site mutations

failure to correctly splice the protein

19

frameshift mutation

addition or deletion causes change in reading frame and abnormal protein

20

how is amino acid linked to tRNA

carboxyl group of AA is in ester linkage with adenosine of CCA at 3' end

21

anticodon

complement of codon in mRNA

22

codon

specifies amino acid needed for the sequence

23

responsible for attachment of AA to tRNA

aminoacyl-tRNA synthetase

24

mediators of circularization of mRNA preventing use of incompletely processed mRNA in translation

eIF-4 proteins, poly-A binding proteins

25

where do eIF-4 proteins bind?

5' cap

26

prokaryotic ribosomal components

70S = 50S + 30S

27

eukaryotic ribosomal components

80S = 60S + 40S

28

[T\F] ribosomal proteins are more abundant in eukaryotic ribosomes

T

29

products of RER ribosomes

proteins for export

30

products of cytosolic ribosomes

proteins for the cell's own use