metabolism 3: Oxidative Phosphorylation Flashcards
For 1 glucose molecule, glycolysis and TCA cycle yields:
10x NADH (and 10H+) and 2x FADH2 (reducing agents)
Each one will carry two high energy electrons
what carries and store electrons for oxidative phopsphorylation
reducing agents
Electrons from NADH and FADH2 reduce O2 to
H2O
In oxidative phosphorylation, the electron transfer potential of NADH+ and FADH2 is converted into
the phosphoryl transfer potential of ATP
Phosphoryl transfer potential:
ΔG during ATP hydrolysis
Electron transfer potential:
measured by redox potential of a compound (E’0) - how easily it donates electrons
Negative (E’0) means
the reduced form of compound has a lower affinity for electrons than hydrogen (+ vice versa)
Oxidative phosphorylation
Produce ATP by donating electrons to complexes in membrane
Cytrochromes:
proteins w haem groups
Electrons from Malate-asparate shuffle:
enter from NADH
Electrons from Glycerol-3-phosphate:
enter from FADH2
Respiratory chain:
electrons transfered from carriers (w positive potentials), O2 → H2O
Oxygen =
final electron acceptor
- Respiratory complexes 1,3, and 4 pump H+ ions into
intermembrane space of mitochondria
- Creates proteon gradient
low of H+ back into matrix through
ATP synthase phosphorylates ADP → ATP
