Mice Flashcards
(100 cards)
1
Q
What is the common abbreviation for C57BL/6 and C57BL/10 strains of mice
A
B6
B10
2
Q
Name a common lesion of the right ventricle in BALB/c mice
A
dystrophic epicardial mineralization
also may develop myocardial degeneration and auricular thrombosis
3
Q
Which strain of mice is most resistant to spontaneous amyloidosis?
A
BALB/c
4
Q
What coat color is the mouse strain C3H/He?
A
agouti
5
Q
What is the mutation that renders C3H/He blind?
A
rd1
Pde6brd1
6
Q
C3H/He are susceptible to which virus that renders them susceptible to a relatively high incidence of mammary neoplasia
A
Murine Mammary Tumor Virus
7
Q
Which strain of mice is the most frequent strain of embryonic stem cells (ES)?
A
129
8
Q
Which strain of mice are renown for testicular teratoma (embryonal carcinomas)
A
129/sv
9
Q
Which strain of inbred Swiss mice are prone to seizures?
A
FVB/N
they are also blind due to homozygosity of the rd1 allele
10
Q
Outbred swiss mice are prone to what ailment that is a major life-limiting disease
A
amyloidosis
they also have retinal degeneration
11
Q
What is the predominate circulating leukocyte of the lab mouse
A
lymphocytes
make up 3/4 of total diff count
12
Q
T or F. Mice are coprophagic?
A
T. They get about 1/3 of their dietary intake from feces
13
Q
Do male or female mice have larger submandibular salivary glands?
A
Males
14
Q
What type of placenta do mice have
A
hemochorial
15
Q
T or F. Proteinuria is lethal in mice
A
False. It is normal! Highest levels noted in mature males
16
Q
T or F. Adult mice have a thymus
A
True. Thymus does not involute in mice
17
Q
T or F. The splenic red pulp of mice is an active site of hematopoiesis, throughout life
A
T
18
Q
Is maternal IgG is transferred in utero or postnatally?
A
both! postnatally by IgG receptors in the SI that actively acquire IG up to 2 weeks
19
Q
Immune response between mouse species is variable. What is a great example of the regarding the immune response of Balb/s vs C57BL/6
A
Ball/c- TH2
C57- TH1
(maybe why C57 are more resistant to many viruses)
20
Q
On what chromosome is the MHC (H-2) complex located in mice
A
17
21
Q
How many lung lobes do mice have
A
1 left
4right lobes
22
Q
Name a melanotic mouse strain
A
B6
many mice strains have melanosis in many organs
23
Q
Describe mouse adenovirus
A
DNA virus, no envelope replicates in the nucleus intranuclear inclusions MAdV-1 and MAdv-2 No natural disease wit MAdv-1 MAdV-2- enterotropic, minimal disease
24
Q
Which 2 herpesviruses affect mice?. What type of herpesvirus are they
A
Mouse cytomegalovirus (MCMV)
Mouse thymic virus (MTV)
betaherpesviruses
25
Describe the hallmark of disease with MCMV
cytomegalic inclusion disease- enlarged cells bearing intranuclear and intracytoplasmic inclusion bodies, in the salivary glands
26
What are the characters lesions of Mouse Thymic virus
intranuclear and intracytoplasmic inclusion bodies and necrosis of thymocytes
27
Name 2 viruses in the family parvoviridae that affect mice
Minute minute virus of mice
| Mouse parvovirus-1
28
What are the characteristics of parvovirus
small single-stranded DNA
29
What are the clinical signs associated with natural infection of MVM
none
30
What is the critical impact of infection with MPV?
significant immune perturbations and aberrations
31
In what stage of the cell cycle are parvovirus likely to replicate
S phase
| replicate in rapidly dividing cells
32
What is the tissue tropism of MPV
lymphoid tissues
33
To what family does the Ectromelia virus belong?
| What are the characteristics of this virus family?
Poxviridae, genus Orthodox
| Large DNA virus
34
T or F. Ectromelia is highly contagious
F
35
What is the suspected route of transmission of ectromelia?
cutaneous trauma, invasion through skin, local replication, spread to regional lymph nodes, viremia, replication in spleen and liver, 2nd viremia (3-4dayspost exposure)
36
Name 2 species of mice that are remarkably resistant to ectromelia
C57BL/6 and AKR
37
What rodent species is the natural reservoir for LCMV
Mus musculus
38
To what viral family doe LCMV belong
Arenavirus
| RNA
39
What common laboratory rodent species appears to be refractory to natural infection with LCMV?
rats
40
What mouse strain is disease resistant to LCMV and why
C3h/He, due to H-2k/k haplotype
41
To what viral family does lactate dehydrogenase virus belong
arterivirdidae, RNA
| frequent contaminate of transplantable tumors
42
What is the significant impact of LDV infection of mice on research
-alteration of macrophage function
43
Describe the characteristics of Mouse Hepatitis Virus
Large enveloped RNA virus, radial spikes (peplomers) that are antigenically distinct between strains
44
What are the clinical signs typically associated with MHV
runting, inappetence (no milk spot) diarrhea, dehydration in neonates (less than 2 weeks)
45
List the primary tissue tropisms of MHV
```
Enterotropic strains
Respiratory strains (polytropic)
```
46
What are the hallmark gross and histologic lesions of MHV
Gross- yellow/white nodules in multiple organs
| Hits- focal necrosis (liver) with syncytial cells on the periphery of the lesion
47
T or F. Serology is useful for detecting and surveillance of infection with MHV
T. Serology is great
| Fecal PCR can be used as well
48
What differential diagnosis should be considered in relation to MHV
Tyzzers, EDIM, Salmonellosis, Ectromelia
49
Impact of MHV on research?
Immune modulation
50
How is MHV treated or managed
- burnout, cessation of breeding for 6-8 weeks
| - Rederivation by c-setion or embryo transfer
51
What viral family does Sendai Virus (SV)belong to
paramyxyoviridae
| closely related of parainfluenza virus-1 of human
52
What other species of rodents are susceptible to SV
rats and hamsters
53
T or F. Sendai virus is highly contagious
True
54
What are typical gross and histologic lesions associated with SV
plum colored consolidated lung lobe(s)
| -necrosis inflammation of nasal and airway epithelium
55
What are the clinical signs associated with SV in mice
dyspnea, chattering, crusting of eyes
| suckling mice more affected than adults
56
Which strains of mice are most resistant to SV
C57Bl/6, AKR, SJL and Swiss
| DBA,129 and C3H most susceptible
57
T or F. SV virus is highly labile
T
58
To what family does Mouse Encephalomyelitis belong
Picornoviridae-small RNA virus
aka Theilovirus (Theiler's virus)
aka Mouse polio
59
In what cell type does MEV (Thriller's virus) replicate
enterocytes- though typically intestinal lesions are not present
60
Describe the the histologic lesion of MEV in SCID mice
cytoplasmic vacuolization of neurons and cells in the neuropil
61
To what viral family does Epizootic Diarrhea of Infant Mice belong (EDIM)
Reoviridae
Rotavirus (Rotavirus A)
-Double stranded RNA!!!
62
T or F. EDIM is highly contagious
T. It is highly contagious and prevalent, though disease is relatively rare once it is enzootic in a population
63
In what cell or tissue does EDIM replicate?
Intestinal mucosa
Infects terminally differentiated enterocytes (infant mice have more of these than older aged animals, which makes them the most susceptible)
64
Clinical signs of EDIM
- yellow mustard colored diarrhea, yet continue to suckle
- transient
- rarely clinically relevant in enzootic populations
65
To what family does Norovirus belong?
Caliciviridae
-non-enveloped RNA
(notoriously stable in the environment!)
66
What is the cellular tropism for norovirus?
Macrophages ans dendritic cells
67
Describe the difference in endogenous versus exogenous retrovirus (MMTV , MLV) transmission
exogenous- transmitted horizontally, through milk
| endogenous- inherited through the genome
68
What is the causative agent of Tyzzer's disease in mice
Clostridium piliforme
69
What is the longevity of Clostridium piliforme spores in the environment?
up to 5 years
maintain infectivity at least 1 year at room temp
Exposure is primarily through ingestion of spores from the environment
70
What strain of mice are most susceptible and most resistant to Tyzzer's disease?
DBA/2- susceptible
| B6- resistant
71
Resistance of B6 mice to Tyzzer's has been attributed to what cell type
B cells
72
What cell type does Mycoplasma pulmonic typically colonize?
Apical cell membrane of respiratory epithelium
73
List some of the clinical signs associated with Mycoplasma pulmonis in mice
weight loss, dyspnea, chattering
74
Are mice or rats more susceptible to Mycobacterium pulmonis?
Rats
75
Describe the characteristics of the Mycobacterium pulmonic organism
pleomorphic, gram-neg- no cell wall,
76
What is the etiologic agent of Transmissible murine colonic hyperplasia
Citrobacter rodentium
| gram negative rod
77
How is Citrobacter rodentium typically transmitted to mice
through contaminated food and bedding
78
What are the two species of Salmonella
S. bongori (mainly infects poikilotherms)
| S. enterica
79
What are the salmonella serovars likely to infect mice (though uncommon)
S. Typhirium
| S. Enteritidis
80
Pneumocystis murina is known to cause severe lung pathology in immunocompetent mice. T or F
F- dz is subclinical in immune competent animals, causes severe disease in immune deficient animals (rubbery lungs that fail to deflate)
81
Describe the organism Spironucleus muris
flagellated protozoa, smaller than Giardia, elongated and pear shaped, lacks an anterior sucking disk
-inhabits the duodenum (crypts of Lieberkahn)
82
Hymenolepis nana is also know as the dwarf tapeworm. T or F
T
| Adults are very small (25-40mm)
83
T or F. The life cycle of Hymenolepis nana requires an intermediate host.
F.
It can have a direct (no intermediate host) or indirect (arthropod intermediate host) life cycle. It is the only known cestode that does not require an intermediate host.
84
How long is the lifecycle of Hymenolepis nana from ingestion to patency?
20-30 days
85
What is genus and species of the common mouse pinworm
Syphacia obvelata
86
Does the life cycle of Syphacia require a host?
No it is direct.
| Completed in 11-15d
87
Which has a longer life cycle?
| Syphacia or Aspicularis
Aspicularis - 23-25 days
| Syphacia 11-15d
88
What is the common pinworm of rats, and how is it differentiated from Syphacia obvelata
Syphacia muris- common pinworm of rats,
| eggs of S. muris are much smaller than obvelata
89
Which inbred strains of mice are most likely to have calcification of the heart?
DBA, C3H, Balb/c
90
Which inbred strains of mice are most likely to have acidophilic macrophage pneumonia?
C57BL/6
91
Hypocallosity is a forebrain commissure defect with aplasia of the corpus collosum. It is common in which 2 inbred strains of mice
129 strains and Balb/c
92
Hydrocephalous is common in which inbred strain of mice?
C57BL
93
Which strains of mice are known to develop a high incidence of thymic lymphoma
SCID, AKR, C58
94
Which inbred strain of mice almost 100% incidence of mammary tumors (by 9 months of age)
C3H
95
What is the most likely diagnosis in a aged mice with unilateral protrusion of the eye.
Hardarian gland adenoma or adenocarcinoma
96
On what mouse chromosome is the Major Histocompatibility complex located?
17
97
T or F
| Each strain of inbred mice have unique H2 haplotypes
They are homozygous
each strain is unique
C57BL- H2b
Balb H2d
98
What is the first inbred strain of mice and who developed it?
DBA
| C.C. Little
99
T or F
| Many Swiss mice are blind dues to homozygous recessive rd1 allele.
T
| This is evidence that Swiss mice are a little bit inbred, even though they are considered an outbred strain
100
T or F
| N-ethyl-N-nitrosurea (ENU) induces random point mutations
T
as does ethylmethansulphate (EMS)
both are given to MALE mice to induce mutations
