Michaelis Menten Kinetics

Question 1

Why is kinetics needed

Answer 1

Question 2

Draw a Michaelis menten plot

Answer 2

Question 3

What does the Michaelis menten equation assume

Answer 3

Also assumes that the reverse reaction( [ES]→ [E]+[S] (k-1) ) can be neglected

Question 4

What is the expression for the disocciation constant

Answer 4

Question 5

Recall the Michaelis menten equation

Answer 5

Question 6

derive the Michaelis menten equation for initial velocity from the expression for the dissociation constant

Answer 6

Question 7

what is the steady state assumption and draw the graph for it *

Answer 7

intermediate species in a reaction mechanism remains constant over the time course of the reaction, even though the reactants and products are changing. This means that the rate of formation of the intermediate is equal to the rate of its consumption
Intermediate = ES

Question 8

recall the equation for the Michaelis menten constant involving rate constants

Answer 8

Question 9

how do you find initial velocity of a reaction using the Michaelis menten constant

Answer 9

Question 10

Explain what the Michaelis menten equation shows and how Vmax and Km are obtained from a Michaelis menten plot

Answer 10

Question 11

What does Km tell you

Answer 11

The affinity of the enzyme for the substrate

Question 12

What does it mean if [Substrate]<Km

Answer 12

Question 13

What does it mean if [Substrate]>Km

Answer 13

Question 14

What are the units of Km and velocity

Answer 14

Question 15

What does it mean if an enzyme has a low Km

Answer 15

Question 16

What factors affect Km

Answer 16

Question 17

Write an expression relating Km and Ks

Answer 17

Question 18

What is Km a measure of

Answer 18

Question 19

What does a high Km mean

Answer 19

Question 20

How does Km affect an individuals susceptibility to alcohol

Answer 20

Question 21

What is kcat

Answer 21

Question 22

How do you calculate initial velocity using kcat and Km

Answer 22

Question 23

What is kcat/Km used to measure

Answer 23

The greater the kcat/Km value, the more efficient the enzyme

Question 24

What are the uses of kcat/Km

Answer 24

Question 25

What is a catalytically perfect enzyme

Answer 25

Is when k2>>k1 This means when the formation of [P] from the ES complex is fast compared to breakdown of ES complex back to enzyme and substrate

Question 26

What is a catalytically perfect enzyme limited by

Answer 26

Question 27

How do enzymes achieve catalytic perfection

Answer 27

Question 28

What does Michaelis- menten kinetics assume

Answer 28

Question 29

What does a Michaelis menten plot show

Answer 29

Question 30

What is the equation that is plotted on a lineweaver - burk plot

Answer 30

Question 31

Draw an example of a lineweaver - burk plot and recall the information you can obtain from it

Answer 31

Question 32

What are the disadvantages of using the lineweaver - burk plot to measure kinetic parameters

Answer 32

Question 33

Recall the equation used to produce a hofstee - eadie plot

Answer 33

Question 34

What information can you obtain from a hofstee -eadie plot and what is the disadvantage of using this plot to obtain kenetic parameters

Answer 34

Question 35

What equation is used to plot a Hanes - Woolf plot

Answer 35

Question 36

Draw an example of a Hanes - Woolf plot and what you can obtain from it

Answer 36

Question 37

Why is the hanes-woolf plot the most accurate out of the other plots discussed

Answer 37

Question 38

What's an equation that can be used to calculate Vmax from kcat and total conc. of enzyme

Answer 38

Question 39

What is the principle of Michaelis- menten kinetics

Answer 39

Until the enzyme is fully saturated

Question 40

What is the modified version of the Michaelis-menten equation that includes k-1 (the backwards reaction)

Answer 40

This treats ES as an intermediate

Question 41

What is the equation for the haldane relationship

Answer 41

Km s and Km p are the Michaelis constants for the substrate and product, respectively. Vmax f and Vmax r are the maximum velocities/ rate for the forward and backward reactions respectively

Question 42

What does the haldane relationship tell you

Answer 42

At equilibrium v=0

Question 43

What is the effect of pH on enzymes

Answer 43

Question 44

At acidic, basic and optimum pH what are the charges of the residues on the substrate and the active site

Answer 44

Question 45

According to Michaelis menten kinetics how do enzymes behave at different pHs*(watch vid)

Question 46

What are lonization constants used for*

Answer 46

Ionization constants are used to describe the extent to which a compound, typically an acid or base, dissociates or ionizes in a solvent, usually water

Question 47

What is the effect of temperature on enzymes

Answer 47

Question 48

What are bisubstrate reactions

Answer 48

Question 49

How are substrates characterised using cleland nomenclature

Answer 49

Question 50

What are the names of the mechanism for bisubstrate reactions and the subcategories

Answer 50

Sequential reactions and ping-pong reactions which can be subdivided into ordered and random

Question 51

What is a sequential reaction

Answer 51

Question 52

What is a ping-pong reaction

Answer 52

Question 53

What are ordered and random reactions

Answer 53

Question 54

Which enzyme doesn't follow Michaelis - menten kinetics and why

Answer 54