Microbial Control (15)

Question 1

thermal Death time

Answer 1

population dependant, time to kill all cells

Question 2

Non-Selective Antimicrobials

Answer 2

• eg. target any protein with sulfhydryl, used on non-living.

Question 3

Selective

Answer 3

eg. penicillin affect prokaryote ribosomes

Question 4

Bacteriostatic

Answer 4

Inhibits growth, still alive but waiting

Question 5

bacteriocidal

Answer 5

Kills cells, cells still there but dead

Question 6

Bacteriolytic

Answer 6

Cells lysis

Question 7

Kirby-Bauer

Answer 7

• figure out what antibiotic to use

- discs of antibiotics

Question 8

Antiseptics

Answer 8

• living tissue
• cidals and septics used
• 60-85% alcohol, triclosan, cationic detergents, 3% peroxide, metals
• no for ingestion

Question 9

Disinfectants

Answer 9

• too harsh on living tissue
• some can sterilize
• strong oxidizers

Question 10

Alcohol

Answer 10

Antiseptic & disinfectant (high concentrations)

-

Question 11

Hydrogen Peroxide

Answer 11

Antiseptic

- oxidizing adgent

Question 12

Triclosan

Answer 12

• phenolic disinfectant
• some are resistant
• disrupts cell membrane

Question 13

Chlorine

Answer 13

• disinfectant

- strong oxidizing

Question 14

Ethylene Oxide

Answer 14

• disinfectant

- alkylating agent

Question 15

Antibiotics

Answer 15

• cidal or static

- do lots of things to disrupt cell function and structure

Question 16

Broad-spectrum vs. Narrow spectrum

Answer 16

Tetra-cycline - broad - protein synthesis
penicillin - narrow, graw-positives - cell wall synthesis
polymyxin B - narrow - some gram negatives - disrupts cell membrane

Question 17

What can radiation do?

Answer 17

- Protein Damage
 DNA:
- double-strand breaks
- stray electrons
- hydroxide ions
- hydride radicals