Principals of Genetics (10)

Question 1

RecA

Answer 1

• needed for recombination with single=stranded binding proteins

Question 2

Transformation

Answer 2

• cells need to be “competent” - salts or electricity

- do this for nutrition, genetic variation, repair

Question 3

Generalized Transduction

Answer 3

• rare occurance, would only contain host DNA

Question 4

Specialized transduction

Answer 4

• specific site of insertion, may pick up genes near the insertion point
• every transducing particle would contain host DNA and phage DNA

Question 5

Episomes

Answer 5

plasmids that can integrate into chromosome

- includes F plasmid

Question 6

Curing

Answer 6

plasmids can be lost

Question 7

Fimbriae

Answer 7

don’t care

Question 8

TraI

Answer 8

unwinds DNA

Question 9

Have F plasmid

Answer 9

• can make F pilus
• mobilization of DNA (same plasmid or other plasmid)
• alteration of receptors

Question 10

F’

Answer 10

has chomosomal genes on the plasmid from Hfr recombination.

Question 11

Insetion Sequence

Answer 11

• inverted repeats

- encodes transposase

Question 12

Transposons

Answer 12

• two insertion sequences moving out together, have genes in the middle that move with it.
• conservative - original transposon excised fully.
• replicative- it remains in site, a copy is made the moves