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01LMR_MOTHER NOTES > MICROBIO W1-DAY3_ALN > Flashcards

MICROBIO W1-DAY3_ALN Flashcards

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1
Q

Test for ID of gram(+) organisms

A

Catalase test

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2
Q

Catalase +/-:

Staphylococci

A

Catalase +

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3
Q

Catalase +/-:

Streptococci

A

Catalase -

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4
Q

Catalase +/-:

Enterococci

A

Catalase -

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5
Q

Catalase +/-:

Listeria monocytogenes

A

Catalase +

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6
Q

Catalase +/-:

Corynebacteria

A

Catalase +

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7
Q

Test initially used for differentiating between groups of Gram (-)

A

Oxidase test

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8
Q

Gram (-) Oxidase (-) bacilli

A

ESA

Enterobacteriaceae
Stenotrophomonas maltophilia
Acinetobacter spp.

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9
Q

Gram (-) Oxidase (+) bacilli

A

PA

Pseudomonas spp.
Aeromonas spp.

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10
Q

Indole test detects which enzyme?

A

Tryptophanase

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11
Q

OXIDASE REACTION

Vibrio:
Enterobacteriaceae:

A

Vibrio: Oxidase +
Enterobacteriaceae: Oxidase -

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12
Q

Indole test presumptively identifies this bacteria

A

E. coli

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13
Q

E. coli IMVC rxn

A

+ + - -

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14
Q

INDOLE TEST RXN

E.coli

A

+

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15
Q

UREASE TEST (+) ENTEROBACTERIACEAE

A

PPM

Proteus
Providencia
Morganella

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16
Q

Key enzyme of H. pylori

A

Urease

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17
Q

Gram - bacilli associated with peptic ulcer

A

H. pylori

NOTE:
Strong producer of urease that converts urea to ammonia which neutralized acid pH

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18
Q

PYRase (+) Streptococcus group

A

Group A (S. pyogenes)

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19
Q

PYRase (+) Enterococcus grp

A

Group D

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20
Q

The only hippurate hydrolysis (+) Campylobacter

A

C. jejuni

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21
Q

Hippurate Hydrolysis (+)

A

SLC-G

S. agalactiae
L. monocytogenes
C. jejuni
G. vaginalis

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22
Q

Common among S. agalactiae, L. monocytogenes, C. jejuni, G. vaginalis

A

Hippurate Hydrolysis +

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23
Q

Planococcus and Stomatococcus are Micrococcaceae genera that can be found among (human/animals).

A

Animals

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24
Q

Grape-like cocci (in clusters)

A

Staphylocci

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25
Gram + micrococcaceae in tetrads or in sarcinae
Micrococcus
26
BAP contains ___% defibrinated (sheep/horse) blood
5%; sheep
27
Preferred types of defibrinated blood in BAP (Descending order)
1: sheep 2: horse 3: rabbit
28
Type of blood used to demonstrate B-hemolytic Haemophilus
Horse
29
Type of blood used to demonstrate B-hemolytic Haemophilus
Horse
30
Non-specific inhibitors found in Human Blood (not preferred for BAP)
Citrate Dextrose
31
Staph/Strep: Pinhead colonies
Staph NOTE: Staph has larger colonies than Strep.
32
Staph/Strep: Pinpoint colonies
Strep
33
MSA inhibitor
7.5-10% NaCl
34
Gram + cocci capable of growing in presence of 7.5% NaCl
Staphylococci (Halophilic)
35
MSA differentiates Staphylocci based on the fermentation of what carb alcohol?
Mannitol
36
Incomplete hemolysis on BAP
Alpha
37
Identify hemolytic pattern on BAP: Produces green or brown area around colony
Alpha (Incomplete)
38
Complete hemolysis
Beta hemolysis
39
Identify hemolytic pattern on BAP: Clear area around colony
Beta (complete)
40
Identify hemolytic pattern on BAP: No lysis of red cells around colony
Gamma
41
Identify hemolytic pattern on BAP: No change in agar
Gamma
42
Identify hemolytic pattern on BAP: Small zone of alpha surrounded by a zone of B hemolysis after refrigeration
Alpha-prime
43
Identify hemolytic pattern on BAP: Surrounded by two zones of hemolysis
Alpha-prime Inner: alpha Outer: Beta
44
TRUE or FALSE: Alpha prime pertains to double zone of hemoysis of C. perfringens.
FALSE C. perfringens: Inner = beta; outer = alpha
45
Golden yellow colonies on LSS
S. aureus
46
Lemon yellow colonies on LSS
S. citreus
47
Porcelain white colonies on LSS
S. albus (S. epidermidis)
48
MSA pH indicator
phenol red
49
S. aureus on MSA
yellow (MF)
50
S. epidermidis (albus) on MSA
pink (Non-M F)
51
S. saprophyticus on MSA
variable (pink/yellow)
52
What should a culture medium contain to be labeled as "selective"?
Inhibitor
53
What should a culture medium contain to be "differential?"
Indicator
54
TSI pH indicator
phenol red
55
Urease medium pH indicator
phenol red
56
Phenol red at an acidic pH
Yellow
57
Phenol red at an alkaline pH
Red
58
Main test to differentiate Staph vs. Strep
Catalase
59
catalase test rgt
3% hydrogen peroxide
60
positive result for catalase test
effervescence, bubbles
61
Test to differentiate Staphylococcus vs Micrococcus
Modified Oxidase Test
62
Staph vs. Micro Lysostaphin susceptible
Staph
63
Staph vs. Micro Furazolidone susceptible
Staph
64
Staph vs. Micro Lysostaphin resistant
Micro
65
Staph vs. Micro Furazolidone resistant
Micro
66
Staph vs. Micro Bacitracin resistant
Staph
67
Staph vs. Micro Bacitracin susceptible
Micro
68
Staph vs. Micro Mod. Oxidase +
Micro
69
Staph vs. Micro Mod. Oxidase -
Staph
70
Staph glucose utilization
Fermenter
71
Micrococcus glucose utilization
Oxidizer
72
Gram + cocci that ferments glucose
Staph
73
Microdase test + reaction for gram + cocci
Blue
74
Modified Oxidase test rgt
Tetramethyle paraphenylene diamine dihydrochloride w/ dimethylsulfoxide (DMSO)
75
Original oxidase (for Neisseria) + reaction
Purple color
76
Glucose utilization test is also known as:
Oxidation-Fermentation (OF) test
77
OF test medium
OF tube with incorporated glucose
78
CHO in OF test
Glucose
79
OF test pH indicator
Bromthymol blue
80
OF test for gram + cocci: Open tube (+) Closed tube (-)
Micrococcus
81
OF test for gram + cocci: Open tube (+) Closed tube (+)
Staphyloccus
82
In OF test, closed tube is sealed with?
Mineral oil or Vaseline with parafilm (VASPAR)
83
Common pH indicators used for OF test
Bromcresol purple (purple to yellow) Andrade's Acid Fuchsin Indicator (pale yellow to pink) Phenol Red (red to yellow) Bromthymol blue (green to yellow)
84
The mechanism of Andrade's Acid Fuchsin indicator is similar to what pH indicator?
Neutral red A: red to pink K: red to yellow
85
Single most important pathogenic determinant of S. aureus
Coagulase Test (S. aureus vs. Coagulase - Staph)
86
Coagulase test rgt
Rabbit's plasma
87
Anticoagulant of choice in using human plasma for coagulase test
EDTA NOTE: Citrate = false (+)
88
Tests used to differentiate coagulase (+) staphylococci
Voges-Proskauer test PYR test
89
Test to differentiate S. epidermidis vs. S saprophyticus
Novobiocin Resistance test

01LMR_MOTHER NOTES (11 decks)

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