Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

Microbiology and Hematology > Microbiology Lab Practical > Flashcards

Microbiology Lab Practical Flashcards

1
Q

What is Body Temp?

A

37° F

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is Room Temp?

A

25°F

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is a broth?

A

a fluid medium for bacterial growth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is an agar?

A

a solid gel-like form in a plate for bacteria to grow on

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is an agar deep?

A

a solid agar material in a tube

no slant

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is an agar slant?

A

a solid agar material in a tube made at a slant in a tube

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is aseptic technique?

A

aseptically transfer bacteria from one source to another without breaking sterility

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is a gram stain?

A

a series of stains that has a specific order and has specific amounts of time for each stain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What stains do you use in a gram stain?

A
  1. Crystal Violet
  2. Grams Iodine
  3. Acetone
  4. Safranin Red
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What does a gram stain tell you?

A
  1. morphology
  2. G +/-
  3. arrangement
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is a negative stain?

A
  • use India ink

- only stain you don’t heat fix

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What does a negative stain tell you?

A

morphology only

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What stains are used in an endospore stain?

A
  1. Malachite Green
  2. Alcohol
  3. Safranin Red
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What does an endospore stain tell you?

A

helps determine whether bacteria is a spore-forming bacteria or not

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What type of bacteria G +/- are endospores usually?

A

G (+) rod

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is a capsule stain used for?

A

can see if the bacteria has a capsule or not

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

What stain do you use in a capsule stain?

A

India ink

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What is a counterstain?

A

a stain meant to help make something else stand out better

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What does gram variable mean?

A
  • both G(+) and G(-)
  • pink G(-)
  • blue G(+)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What is an acid fast stain used for?

A

good for bacteria that has a lipid (fatty) cell wall which can be difficult to stain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

What is selective media?

A

allows growth of specific organism

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

What are differential media?

A

used to distinguish one organism from another

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

What is a lawn of bacteria?

A

creating a solid sheet of bacteria on a plate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

What does bacteriostatic mean?

A
  • stops the growth of bacteria

- doesn’t kill bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Where is the bacteriostatic range on a plate with antibiotics?
the edge of the circle where an antibiotic has killed bacteria
26
What does bactericidal mean?
kills bacteria
27
Where is the bactericidal range on a plate with antibiotics?
ring around the antibiotics where there is no bacterial growth
28
How can you measure the bactericidal range?
- with a ruler | - in mm
29
What is serial dilution used for?
to dilute one ml of a sample to grow countable number of colonies
30
What is a vegetative cell?
an inactive cell
31
What is an endospore?
a vegetative cell that can form a spore
32
What is an obligate aerobe?
needs oxygen to survive
33
What is an obligate anaerobe?
can't survive in oxygen
34
What is a facultative anaerobe?
- can live with or without oxygen | - prefers oxygen
35
What is a bacterial capsule?
a thick glycocalyx shell around a bacteria
36
What does an alpha hemolysis do?
- partial hemolysis | - hazy halo around growth
37
What does a beta hemolysis do?
- complete hemolysis | - clear halo around growth
38
What does a gamma hemolysis do?
- no hemolysis | - grows on top of agar
39
What is a catalase test used for?
Designed to detect bacterial respiration producing the "catalase" enzyme
40
What does a positive catalase test look like?
will have bubbles
41
What is an oxidase test used for?
Designed to detect bacterial respiration producing the enzyme "cytochrome oxidase"
42
What does a positive oxidase test look like?
will turn purple
43
What is a bromcrestol slant used for?
Determines if bacteria can utilize glucose for nutrient growth
44
What does a positive bromcrestol slant look like?
will turn yellow
45
What are phenyl red glucose, sucrose, and lactose tests used for?
* Determine if bacteria can utilize the carbohydrate glucose, lactose, or sucrose * Has a "durham" tube to show whether or not bacteria is growing because gas will be produced
46
What does a positive phenyl red glucose, sucrose, and lactose test look like?
- will turn yellow from red | - durham tube will have a gas bubble if bacterial growth
47
What is a simmons citrate agar used for?
Determines if the bacteria can utilize sodium citrate (carbon) as a source for survival
48
What does a positive simmons citrate test look like?
will turn brilliant blue
49
What is a urea agar test used for?
Determines if bacteria contains an enzyme called "urease"
50
What does a positive urea agar test look like?
color will change to a brilliant pink
51
What is a MRVP broth made up of?
1. MR: methyl red | 2. VP: voges proskauer
52
What is a Methyl Red (MR) used for?
Reagent designed to detect organisms that can overcome a phosphate buffer and can lower the pH of an environment
53
What does a positive Methyl Red (MR) test look like?
turns very red
54
What is a voges proskauer test used for?
Reagent designed to detect bacterial fermentation that produces "acetoin"
55
What does a positive voges proskauer test look like?
will have a really dark ring of red-brown at the top of the tube
56
What does a sims tube test used for?
Provides ability to determine 3 characteristics of a single bacteria
57
What does a positive sims test look like?
Indole: red section at top of tube Motility: cloudy agar Sulfur: black
58
What is a TSA plate used for?
everything, anything
59
What is a glucose min. salt plate used for?
salt and sugar loving bacteria
60
What does a glucose min. salt plate promote?
G(-)
61
What does an EMB plate promote?
G(-)
62
What does an EMB plate inhibit?
G(+)
63
What does a PEA plate inhibit?
G(-)
64
What does a PEA plate promote?
G(+)
65
What does an Endo plate promote?
G(-)
66
What does an Endo plate inhibit?
G(+)
67
What is a mannitol salt plate used for?
salt loving bacteria
68
What does a MacConkey plate inhibit?
G(+)
69
What does a MacConkey plate promote?
G(-)
70
What plates have lactose?
1. EMB 2. Endo 3. MacConkey
71
What plates can grow/show coliforms?
1. EMB 2. Endo 3. MacConkey
72
Does E. coli and/or S. aureus grow in a 2% salt solution?
both
73
Does E. coli and/or S. aureus grow in a 5% salt solution?
S. aureus
74
Does E. coli and/or S. aureus grow in a 8% salt solution?
S. aureus
75
Does E. coli and/or S. aureus grow in a 11% salt solution?
S. aureus

Microbiology and Hematology (20 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions