Microorganisms Staining

Question 1

Define: Bacteria

Answer 1

Single cell organisms

Question 2

Define: Cocci

Answer 2

Spherical or spheroid bacteria

Question 3

Define: Bacilli

Answer 3

Rod shaped bacteria

Question 4

Define: Spirochetes

Answer 4

Spiral or corkscrew shaped argyrophilic bacteria

Question 5

Define: Mycobacteria

Answer 5

A genus of slender, rod-shaped bacteria. Included are the causative organisms of tuberculosis and leprosy

Question 6

Define: Fungi

Answer 6

Primitive plants that possess no roots, stems, leaves, or chlorophyll

Question 7

Define: Yeasts

Answer 7

True fungi, usually single celled, oval shaped fungi that reproduce by budding at the smaller end of the cell

Question 8

Define: Protozoans

Answer 8

Single celled animals that are simple structurally but complex functionally

Question 9

Define: Hyphae

Answer 9

Vegetative, tubular fungal structures that may or may not possess cross walls (septa)

Question 10

Define: Mycelia

Answer 10

Fungal colony composed of a mat of intertwined hyphae

Question 11

Acid-fast techniques:
1. Substance demonstrated:
2. Preferred fixative:
3. Primary reagents & their purpose:
4. Results of the stain:
5. Control material:

Question 12

Gram stain modifications:
1. Substance demonstrated:
2. Preferred fixative:
3. Primary reagents & their purpose:
4. Results of the stain:
5. Control material:

Question 13

Giemsa:
1. Substance demonstrated:
2. Preferred fixative:
3. Primary reagents & their purpose:
4. Results of the stain:
5. Control material:

Question 14

Periodic acid-schiff (PAS):
1. Substance demonstrated:
2. Preferred fixative:
3. Primary reagents & their purpose:
4. Results of the stain:
5. Control material:

Question 15

Chromic acid-Schiff (CAS):
1. Substance demonstrated:
2. Preferred fixative:
3. Primary reagents & their purpose:
4. Results of the stain:
5. Control material:

Question 16

Grocott modification of Gomori methenamine-silver:
1. Substance demonstrated:
2. Preferred fixative:
3. Primary reagents & their purpose:
4. Results of the stain:
5. Control material:

Question 17

Mayer mucicarmine:
1. Substance demonstrated:
2. Preferred fixative:
3. Primary reagents & their purpose:
4. Results of the stain:
5. Control material:

Question 18

Warthin-Starry:
1. Substance demonstrated:
2. Preferred fixative:
3. Primary reagents & their purpose:
4. Results of the stain:
5. Control material:

Question 19

Dieterle:
1. Substance demonstrated:
2. Preferred fixative:
3. Primary reagents & their purpose:
4. Results of the stain:
5. Control material:

Question 20

Steiner & Steiner:
1. Substance demonstrated:
2. Preferred fixative:
3. Primary reagents & their purpose:
4. Results of the stain:
5. Control material: