Microscope Flashcards

(12 cards)

1
Q

What are the rules for biological drawing?

A

Observe the specimen and record what you see clearly and accurately. Labelled drawings and diagrams can be used.

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2
Q

What are some points you need to remember when observing through a microscope?

A
  • Draw what you see, not what you think you should be seeing.
  • Always use a sharp pencil
  • Make your drawings large
  • Make the lines clear and sharp
  • Do not shade or colour in any areas
  • Make sure the different parts of the drawing are in proportion
  • Labels should be spaced neatly around the drawing
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3
Q

What is the difference between electron and light microscopes?

A

Electron microscopes have a higher resolution and magnification than light microscopes.

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4
Q

What is magnification?

A

Magnification is how much bigger a sample appears to be under the microscope than it is in real life.

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5
Q

What is resolution?

A

Resolution is the ability to distinguish between two points on an image i.e. the amount of detail

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6
Q

Pros and cons of electron microscopes

A
  • Expensive to buy
  • Expensive to produce electron beam.
  • Large and requires special rooms.
  • Lengthy and complex sample prep.
  • Preparation distorts material.
  • Vacuum is required.
  • All images in black and white.
  • Magnifies over 500 000 times.
  • Specimens are dead, as they must be fixed in plastic and viewed in a vacuum
  • The electron beam can damage specimens and they must be stained with an electron-dense chemical (usually heavy metals like osmium, lead or gold).
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7
Q

Pros and cons of light microscopes

A
  • Cheap to purchase
  • Cheap to operate.
  • Small and portable.
  • Simple and easy sample preparation.
  • Material rarely distorted by preparation.
  • Vacuum is not required.
  • Natural colour of sample maintained.
  • Magnifies objects only up to 2000 times
  • Specimens can be living or dead
  • Stains are often needed to make the cells visible
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8
Q

What are the main limitations of the electron microscope?

A

The inability to observe living specimens, difficulty of preparation of samples and expensive equipment are the main limitations.

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9
Q

What are the main limitations of the light microscope?

A

Low resolution and as a result cannot magnify above x1500 and still give a clear image

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10
Q

What is cell division? (mitosis)

A

The division of a cell into two daughter cells with the same genetic material.

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11
Q

Magnification

A

x 1000 x1000
mm μm nm

Example: 1mm 1000μm 1,000,000nm

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12
Q

Equations

A

Actual size = Image size ➗ Magnification

Magnification= Image size ➗ Actual size

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