mirco Flashcards

(56 cards)

1
Q

giemsa stain

A

Ricky got Chlamydia as he Tried to Please the Bored Hot geisha

rikettsia
trypanosomes
chlamydia
borrelia
helico
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2
Q

acid shiff stain

A

whipple disease

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3
Q

ziel neelsen

A

acid fast, mycobacterium, nocardia,crytosporidium oocyst

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4
Q

india ink

A

cryptcoccus

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5
Q

siver stain

A

pneumocystis, legionella, helico

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6
Q

diptheria

A

inact. EF2

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7
Q

pseudomonas

A

inact. EF-2

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8
Q

shigella mech. and contraction

A

shigatoxin, inact. 60s by removing adenine from rRNA—-> HUS

4F’s Fingers, Food, Flies, Feces invasion of M-cell

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9
Q

EHEC

A

shigalike toxin, nact. 60s by removing adenine from rRNA—->serotype 0157:H7 does not invade the cells

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10
Q

ETEC

A

watery diarrhea: “labile in Air (Adenyle cyclase), stable on the Ground (guanylate cyclase)

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11
Q

the bacteria that increase cAMP

A

CAPE

cholera
anthracis
pertussis
E.coli

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12
Q

whooping cough

A

b. pertussis

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13
Q

clostridium tetani mech of action?

A

Tetanospasmin that ia a proteases that cleaves SNARE,, the toxin prevents the release of GABA

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14
Q

clostridium botulinum mech of action?

A

Botulinum toxin that is a proteases that cleaves SNARE,, the toxin prevents the release of ACh

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15
Q

Gram stain

A

first line lab test to inden. bacteria with thick petidoglycan layer crytal violet dye (garm +) and bact. with thin peptidoglycan layer turn red or pink (gram-) with counterstain.

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16
Q

Flurescent antibody stain

A

Used to identify many bacteria, viruses, pcp, giardia and crypto. eg FTA-ABS for syphilis

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17
Q

Chocolate agar is for?

A

H. flu

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18
Q

Thayer-Martin is for?

A

N. gonor., N mening. contians: Vanco, Trimethroprim and Colistin and Nystatin.

Very Typically Cultures Neisseria

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19
Q

Bordet is for?

A

Berdet for Bordetella

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20
Q

Löffler is for?

A

C. diph

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21
Q

Löwenstein is for ?

A

TB

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22
Q

Eaton is for?

A

M. pneumo

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23
Q

Mac Conkey agar is for?

A

lactose Fermenting, KEES

24
Q

EMB (Eosin-methylene blue) is for?

25
Charcoal yeast buffered with cysteine and iron is for?
Legionella -ella, brucella, fancisella
26
Urease- positive organism
Pee CHUNKSS ``` Proteus Crytococcus H. pylori Ureapasma Nocardia Klebsiella S. epidermis ```
27
Catalase-positive
Cats Need PLACESS to Belch their Hairballs Catalase degrades H2O2 into H2O+O2 Norcardia, Pseudomonas, Listeria, Aspergillus, Candida, Ecoli, Staphylococci, Serratia, B cepacia, H.pylori
28
yellow sulfer granules is produced by?
Actinomyces israelii
29
yellow pigment
S. areus
30
green pig.
Pseudom.
31
red pig.
Serratia for Sirene
32
Biofilm producing?
S. epi, S. viridans (S mutans, S sanguinus), Pseudomonas
33
What is the virulence factor of S. aureus?
Protein A Binds Fc of IgG. Prevents opsonization and phagocytosis. Expressed by S. aureus
34
What is the virulence factor of SHiN?
IgA protease Enzyme that cleaves IgA, allowing bacteria to adhere to and colonize mucous membranes. Secreted by S. pneumo, H.flu type b, Neisseria
35
What is the virulence factor of group for Strep A.?
M. protein helps prevent phagocytosis. Expressed by the S. group A. Shares similar to human cellular proteins (molecular mimicry), possibly underlines the autoimmune response seen in acute rheumatic fever
36
Costridium deficile exotoxin?
alpha toxin phospholipase (lecithinase) that degrades tissue and cells mambrane-----> myonecrosis (gas gangrene) and hemolysis (doubel zone on blood agar)
37
Stretococcus pyogenes toxin?
Streplysin O protein that degrades cell membrane-----> lyses RBS, beta-hemolysis; host antibodieas aganst toxin (ASO) is used to diag. rf
38
S. areus toxin?
TSS toxin-1 Cross-links beta region of TCR to MHC class II on APCc outside of the antigen binding site ---->Overwelming rel release of IL1,2,gamma and TNF alpha------>schock
39
S. pyogenes toxin?
Erythrogenic exotoxin A Cross-links beta region of TCR to MHC class II on APCc outside of the antigen binding site ---->Overwelming rel release of IL1,2,gamma and TNF alpha------>schock Toxic shock-like syndrome
40
Endotoxins
LPS found on the outer membrane of gram-neg. Composed of O antigen+ core polysaccharide----->lysis of cells 3 effect of: amcrophage activation, complement activation and tissue factor ENDOTOXINS: Edema, Nitric oxide, DIC/Death, Outer membrane, TNF-alpha, O-antigen+Core polysacc.+lipidA, eXtreme heat stable, IL1 and IL6, neutrophile chemotaxis, Shock
41
OVRPS
Optochin Virdans Resitant Pneumoniae Sensitive
42
B-BRAS
Bacitrcin-group B strep are Resistance, grou Strep A sensitive
43
Novobiocin
staph No StRESs: Saprophyticus is Retsistant; Epidermis is Sensitive
44
alpha hemolytic
S. pneumo and Viridan alpha just like OVERPS
45
beta hemolytic
RBC lysis S. areus, S.pyogenes, S. agalac beta sounds like PetAA
46
MRSA
is caused by altered p-proteins (penicillin binding proteins) and the mecA gene
47
bile soluble is?
S. pneumo
48
PYR
GroupA S. pyogenes and enterococcus (faecium and faecalis)
49
stridor
H.flue
50
non-lactose fermenting
SYPS Shigella, Yersenia, Pseudo, Salmonella
51
Oxidase pos.
Pseudo, Campi, Cholera, H.pylori
52
Which Staph is coagulase neg.?
S. Epi
53
Which Staph is coagulase pos.?
S. Aureus
54
wheezing
RSV
55
B. Anthracis toxin and capsule
Gram+, spore f. rod, anthrax toxin/ an exetoxin that produces protective antigen, lethal and edema factor. Has a polysacc. capsule (polyD-Glutamate). Medusa
56
B. Cerus
Gram+, Reheated rice syndrome, enterotoxin