MOD 7 - COURSE 2 - PT 1 - IDENTIFICATION

Question 1

When do individual aniamsl not have to be accounted for/ amrked seperately

Answer 1

• of all same genotype - treated the same - individual variation response not needed to be accounted for seperately

Question 2

what are some important characteristics of identification method

Answer 2

• permenatn, indisputable, no burden on aniaml, letter and number not too complex - reduce human error

Question 3

what is the cage card used for?

Answer 3

• used to identifity mice and rats - identified by cage cards
• ## different colours used - faciliate distinction between different strains, aniamls belonging to different investigators, aniamls usedw with different research protocols - an ddifferent protocol severity levels

Question 4

what info is on the cage card

Answer 4

• complete and correct nomenclatures
• number n dsex of the aniamls and genotypes
• name of the responsible investigator -UKPIL number - and if appropriate the protocol number PPL Section E number - of the research project - for which they will be used plus interventions and dates
• in breeding colonies - cage card = may indicate pedigree number and reprouctive performance history - date mated, birth adn weaning dates of litters or the number of pups borna nd weaned

Question 5

whar are the 2 types of individual identification methods

Answer 5

• temporary method
• permenant methods

Question 6

what are some non-invasive methods of identification

Answer 6

• fur marking
• skin marking
• coat dying

Question 7

what are some invasice methods of idenitfication

Answer 7

• ear tah
• subcutaneous injection

Question 8

what is fur marking

Answer 8

• cutting fut - area on back = cut/shaved
• felt tup pen/ markers - hairless part of body - skin
• coat dying - aniaml have fur
• shaving - only a few different identification
• colours = more options

Question 9

whats an ear tag - how is it used to identification between different animals

Answer 9

• • prenumberes, metal or plastic
• metal - can cause infalmmatory/ absnormal tissue growth
• plastic - 2D barcodes etched on titanium ear tags - attched to plastic tag - red using barcode reader
• ear tags - 4 O’clock position - so tag doesnt fold the ear over or hang down against neck - prediposing anaimls - to ear trauma

Question 10

what is a subcutaneous ink injection wokr to identify aniamsl

Answer 10

• SC injection - differ from tattor -just under skin - no into skin layers
• all SC depost ink - fades over time - few days
• normally footpad or tail - most common site of injection
• can be used all ages
• not reccomeneded - onvasive, painful, temporary

Question 11

are ALL permenant metods of makring anaimls invasive or non invasive

Answer 11

• invasive

Question 12

what permenant identification methods generate tissue samples

Answer 12

• ear punch/ notching
• distal phalanx removal

Question 13

what permenant identification methods dont generate tissue samples

Answer 13

• micrchip
• tattoo

Question 14

what is ear notch/punch nmethod of permenant identification

Answer 14

• easy to carry out- trauma to aniamsl - is iminimal if proprerly executed procedure
• tissue = collected aspetically for genotyping
• location of holes = accurate - according to chart system - to ensure valid identification
• pinna holes can heal

Question 15

what is the distance phalanx remove

Answer 15

• entirre distal phalanx of tip = removed - sharp siccors mousepup - 5-7 days of age
• cut = places - distal part of 2nd phalanx - remove entrire nail bed - avoid regroqth
• if removed correclt y- sidplay little or no raction - mainly paw wthdawal during after the procedure
• not reccomenede rats - due to long term effects - imparied grip sregnth at weaning

Question 16

what is the microchip transponder implantations

Answer 16

• electric radio frequency - ID transponder micrchip - inseted SC 0 neck/further back
• read with transponder reader
• little identification errors
• suitable time for insertion - depend son size of body wirhgt of naimls - rather than age
• long term microchip = cause inflammation - fibrus growth
• appears oto increase risk for developing tumours, and immunoological and skin studies = also infected
• there are new light activation microtransponser - radio frequency - implanted SC - into tail of mice due to small size minimise stress

Question 17

what is tattooing as a method of permenatn identifiacation

Answer 17

• penetration - of skin - intradermenally
• ink = loaded into dermis under epidermis - creat permenet marking
• chemical compound - spread via circulation to entire bdy
• poses type 2 pf hazard - those associated with toxicity - may interefere with study
• severalbody parts - used for tattoo identification in rodents i..e ears, tail, footpads, toes
• ears = sensitive - considering moderatly painful
• methods - required fully developed ears - goos restraint that fixes head of aniaml - need to avoid lateral movement - unncescsary tisiuu edamage during application
• tail tattooing - microtatoor system - applied only in yound animals - ONLY BEFORE osification of tail 2-3 weeks
• needle s- inserted only pads - marked - can identificy all aniamls of all ages
• hwoevr it is consered accpetable - can be adapted to animals of all sizes