Module 1 Flashcards
(15 cards)
How are organisms classified NOT NFB?
Acid is produced anaerobically, in the butt of a TSI, glucose fermentation and in both OF tubes
What is the most commonly isolated species of Aeromonas?
hydrophilia
What are the characteristics of Aeromonas hydrophilia?
Gram neg monotrichous rods
Facultative, 35°C
Beta on BAP, large grey moist, colonies turn dark olive green with age
LLF on MAC
CIN- red centres (same as Yersinia)
What are the ID tests for Aeromonas hydrophilia?
Ox pos
Vitek/MALDI-TOF/API
DNase pos
What is the significance of Aeromonas hydrophilia?
Aquatic, found normally in freshwater, fish, snakes, frogs, soil, food
May show no affect when present in stool or may cause watery diarrhea
What extraintestinal infections are caused by Aeromonas hydrophilia?
Cellulitis and wound
Septicaemia- immunosuppressed
Miscellaneous- UTI, peritonitis, meningitis, osteomyelitis
What are the characteristics of Plesiomonas shigelloides?
Similar to Shigella
Gram neg rods with lophotrichous flagella
BAP- gamma, grey moist
MAC- NLF or LLF
What are the IF tests for Plesiomonas shigelloides?
Ox pos
Vitek/MALDI-TOF/API
Biochem tests- diff from Aeromonas but similar to Shigella
May react with group D antisera (sonnei)
What is the significance of Plesiomonas shigelloides?
Found in surface water
Infection from contaminated food or water
Mild watery diarrhea
Self limiting
Can be severe in children or the immunosuppressed
What are the characteristics of Vibrio?
Straight or curved gram neg rods with polar flagella
Ferment carbs
BAP- translucent, beta or gamma
MAC- NLF
Yellow on TCBS
What are the ID tests for Vibrio cholera?
Ox pos
Vitek/MALDI-TOF/API
DNase pos
What species causes cholera?
Vibrio cholera
What agar is used to rule out Vibrio?
Thiosulfate citrate bile salts sucrose agar (TCBS)
What do the different colours of colonies on TCBS signify?
Yellow- Vibrio
Blue- Pseudomonas or Aeromonas
Small translucent- other Enterobacteriaceae
What is the significance of Vibrio cholera?
Nonsterile water sources
Infection through ingestion of contaminated water or seafood, skin and mucosal surfaces
Fecal-oral route
Colonize small intestine and produce enterotoxin
Severe dehydration
Watery rice water stools with flecks of mucous, vibrios and epithelial cells
