Module 3 Flashcards
(16 cards)
What are the characteristics of Campylobacter jejuni (Campy)?
Gram neg curved rods with variable shapes and one polar flagellum at one or both ends
Pale staining
Tumbling or darting movement
Microaerophilic, 42-45°C
No growth in MAC or other enteric media
What is used to achieve the microaerophilic environment required to grow Campy?
Campy gas paks
Evacuation replacement
5% oxygen, 10% CO2, 85% nitrogen
What are the selective ingredients found in media for Campy?
Base- nutrient, blood, Columbia, Brucella
Blood
Antimicrobials
What media is used to isolate Campy and what are its characteristics?
Campy-BAP
Brucella agar base
10% sheep blood
Vancomycin, trimethoprim, polymixin B, cephalothin, amphotericin B
What other organisms grow on Campy-BAP?
Yeast
Pseudomonas
What do colonies of Campy look like?
Grey-colourless
Gamma
Watery
What are the ID tests for Campy?
Growth on selective media for Campy at 42°C
Ox pos
Hippurate hydrolysis pos (Campylobacter coli neg)
Susceptible to nalidixic acidic, variable susceptibility to cephalothin (Campylobacter coli always resistant)
Doesn’t grow on MAC- diff from Pseudomonas
What is the significance of Campy?
Domestic animals
Infections due to food and water (undercooked poultry)
Most commonly isolated enteric pathogen
Incubation 2-10 days
Mild discomfort followed by pain, bloody diarrhea, chills and fever
Self limiting
Seen in blood cultures
What are the characteristics of Heliobacter pylori?
Gram neg curved rods with polar tuft of flagella coated with a sheath
Microaerophilic, 35°C
Grows on BAP/CHOC, sometimes selective media (MTM), won’t grow with cephalosporins
Colonies- small, grey, translucent, beta or gamma
What is the diagnosis of Heliobacter pylori?
Stomach or duodenum biopsy, not fixed
Touch prep
On urease medium for pos test
What tests can be performed for Heliobacter pylori?
Breath test- radioactive drink of urea, breath analyzed for radioactive carbon or urine for radioactive nitrogen
What can be used to create a microaerophilic environment to grow Heliobacter pylori?
Campy gas pak
How long are Heliobacter pylori specimens incubated for?
Up to 7 days
Seen in 3-5
What are the ID tests for Heliobacter pylori?
Ox pos
Cat pos
Urease pos
What is the treatment of Heliobacter pylori?
Major cause of gastritis and peptic/duodenal ulcers
Only in humans
Urease provides protective alkaline shield in stomach acid
Corkscrew motility allows penetration to epithelial layer
How is Heliobacter pylori treated?
Metronidazole, bismuth and amoxicillin
14 days
