MT2 mechanisms and structures Flashcards
Draw the glycogen phosphorylase mechanism.
Refer to notes
Draw out a pathway for how glycogen phosphorylase gets activated by phosphorylation
Refer to notes
Draw the mechanism for glycogen catabolism from epinephrine secretion.
1 - Epinephrine binds to its specific receptor (alpha and beta)
2 - Occupied beta receptor is a G-protein-coupled receptor. Binding causes displacement of the GDP boudn to Gs by GTP, activating it.
3 - Gs (alpha subunit) moves to adenylyl cyclase, activating it.
4 - Adenylyl cyclase catalyzes the formation of cAMP
5 - PKA activated by cAMP (4 cAMP bind to two reg. subunits to relieve inhibition of catalytic units)
6 - PKA catalytic unit phosphorylates: phosphorylase kinase, glycogen synthase, PP1 (bottom, dominant inactivating) and PP1 inhibitor
7 - cAMP degraded by cyclic nucleotide phosphodiesterase
Draw an arrow and box diagram indicating maintenance of blood glucose levels in the blood by the liver glycogen stores.
Refer to notes
Describe the activation/inactivation of PP1.
Refer to notes.
Draw the PDC mechanism.
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Draw the mechanism (moreso structures) of the top part of the pentose-phosphate pathway. I.e. from G6P to Ribulose-5-phosphate.
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Draw the mechanism for phosphoglucomutase
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Draw the mechanism for a-ketoglutarate DH.
Refer to notes (same as PDC)
Draw the structures, name the enzymes and indicate all substrates for all 8 reactions of the CAC.
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Draw the structure of Oxaloacetate and Acetyl-CoA.
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Draw the structure of Citrate.
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Draw the structure of isocitrate
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Draw the structure of alpha-ketoglutarate.
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Draw the structure of Succinyl-CoA
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