Nonsporeforming, Nonbranching, Catalse (-)

Question 1

Only sp. of Erysipelothrix that cause disease to human.

Answer 1

Erysipelothrix rhusiopathiae

Question 2

3 species that are non-spore-forming, Nonbranching Catalase (-).

Answer 2

Erysipelothrix rhusiopathiae
Arcanobacterium
Gardnella vaginalis

Question 3

Gram+
Catalae-
Pleomorphic
Tend to form long filaments

Answer 3

Erysipelothrix rhusiopathiae

Question 4

Erysipelothrix rhusiopathiae can be acquired through

Answer 4

Occupational exposure (handling fish and animal products)

Question 5

T or F
The route of infection of E. rhusiopathiae is through mouth.

Answer 5

F. - cuts or scratches

Question 6

E. rhusiopathiae is resistant to ____.

Answer 6

Salting, picking, smoking

Question 7

2 common clin. Infection of E. rhusiopathiae.

Answer 7

Erysipeloid
Septicemia

Question 8

Which is not true about erysipeloid?
A. Usually resemble to strep. Erysipelas
B. It contains pus
C. Painful and swollen
D. Sharp and slightly defined
F. Purplish-red zone

Answer 8

B.

Question 9

Disease casue by E. rhusiopathiae that is usually associated with endocarditis.

Answer 9

Septicemia

Question 10

Most common infection caused by E. rhusiopathiae.

Answer 10

Erysipeloid

Question 11

Identify what bacteria
- thin,rod shaped, gram + that can form long filaments. It is arragein single, short chains, or in V shape.

Answer 11

E. rhusiopathiae

Question 12

T or F
E. rhusiopathiae is difficult to decolorized.

Answer 12

F. - easily decolorize.

Question 13

Specimen used in isolation of E. rhusiopathiae.

Answer 13

Tissue
Aspiration in skin

Question 14

Find the mistakes
Inoculate to NA w/ 2% glucose
Incubate in 5% CO2 at 37°C

Answer 14

1% glucose
35°C

Question 15

Appearance of colonies of E. rhusiopathiae in SBA.

Answer 15

Non-hemolytic
Pinpoint

Question 16

After 48hrs of incubation, 2 distinct colonies are form. What are they?

Answer 16

Smaller (smooth, younger)
Larger (rough, older)

Question 17

The hemolysis of E. rhusiopathiae in SBA is ___.

Answer 17

Alpha hemolysis

Question 18

T or F
E. rhusiopathiae is nonmotile, pleomorphic, catalase (-)

Answer 18

True

Question 19

In E. rhusiopathiae, what is the reaction in different biochem tests?
H25
VP
Urease
Esculin hydrolysis

Answer 19

Positive
H25
Negative
VG
Urease
Esculin hydrolysis

Question 20

Appearance of E. rhusiopathiae in gelatine stab culture.

Answer 20

Test tube brush pattern.

Question 21

What are the medical inportant Arcanobacterium ?

Answer 21

A. haemolyticum
A. pyogenes
A. bernardiae

Question 22

An Arcanobacterium sp. that is animalnpathogen and best known for causing infection in cattle

Answer 22

A. pyogenes

Question 23

A. haemolyticum is formely known as __.

Answer 23

Corynebacterium haemolyticum

Question 24

Recovered from 10-20 yrs. Old with pharyngitis

Answer 24

A. haemolyticum

Question 25

Develop pruritic, scarlatiniform rash, desquamation of the skin of hands and feet.

Answer 25

Arcanobacterium haemolyticum

Question 26

What is observed when the colony of A. haemolyticum is remove?

Answer 26

Black opaque dot And pitting of agar

Question 27

A. haemolyticum is both lipase and lecithinase ___.

Answer 27

Positive.

Question 28

Result of A. haemolyticum in CAMP test.

Answer 28

Reverse CAMP reaction the arrow head has no hemolysis

Question 29

Concept of CAMP Test in A. haemolyticum.

Answer 29

It inhibits reverse CAMP rxn. Because the hemolysis produced by a Beta-lysin-producing S. aureus is inhibited by a phoppholipase D excreted by A. haemolyticum

Question 30

Drug of choice for A. haemolyticum.

Answer 30

Erythromycin

Question 31

Short, pleomorphic, gram+ and rod/coccobacillus that often gram-variable or gram(-)

Answer 31

Gardnerella vaginalis

Question 32

T or F Gardnerella vaginalis is the only sp. In this genus.

Answer 32

True

Question 33

Microflora in Female genital tract.

Answer 33

G. vaginalis

Question 34

This infection is only cause by G. vaginalis.

Answer 34

Bacterial vaginalis

Question 35

Describe as malddorous discharge and vaginal ph 7 than 4.5

Answer 35

Bacterial vaginalis

Question 36

Effect of Bacterial vaginalis

Answer 36

Reduction of Lactobacillus Increase of pH Increase G. vaginalis

Question 37

Drug used for Bacterial vaginalis

Answer 37

Metronidazole

Question 38

Large squamous epithelial cells with gram + and gram variable bacilli and coccobacilli clustered in edge in bacterial vaginalis infection is called??

Answer 38

Clue cells

Question 39

___ for gram stain vaginal smear is more accurate compared to culture in diagnosing BV.

Answer 39

Nugent scoring system

Question 40

G. vaginalis grows best at what concentration of CO2 and at what temp?

Answer 40

5-7% CO2 and 35-37°C

Question 41

Medium of choice for G. vaginalis .

Answer 41

Human blood Bilayer Tween (HBT) agar.

Question 42

Appearance of G. vaginalis in HBT agar.

Answer 42

B hemolytic, small, gray, opaque

Question 43

Biochemical reaction of G. vaginalis In Catalase Oxidase Hippurate hydrolysis

Answer 43

Catalase (-) Oxidase (-) Hippurate hydrolysis (+)