Spore-forming, Nonbranching Catalase Positve

Question 1

Gram +, catalase +, aerobic/facultative anaerobes

Answer 1

Bacillus

Question 2

Bacillus can be confused with ___

Answer 2

Clostridium spp.

Question 3

Most medical relevant group of bacillus.

Answer 3

Bacillus cervus group
-B. anthracis
-B. cereus
-B. thuringiensis
-B. mycoides

Question 4

Most important member of B. cereus group.

Answer 4

B. anthracis

Question 5

Vrulence factors of B. anthracis

Answer 5

-Glutamic acid capsule
-anthrax toxin (three-component protein exotoxins) lethal

Question 6

Virulence factors of B. anthracis that code the genes for toxins carried on plasmid

Answer 6

Anthrax toxins

Question 7

This virulence factors of B. anthracis is resistant to hydrolysis by proteolytic enzymes.

Answer 7

Glutamic acid capsule

Question 8

3 proteins of Anthrax.

Answer 8

Protective antigen (PA)
Edema factor (EF)
Lethal factor (LF)

Question 9

T or F
in anthrax toxin protein. Each of which individually is nontoxic but which together act synergistically to cause effect.

Answer 9

True

Question 10

Protein of anthrax toxin that bind molecules and protect EF and LF

Answer 10

Protective antigen

Question 11

Among the 3 proteins of anthrax toxins, which one is adenylate catalase?

Answer 11

Edema factor (EF)

Question 12

Protease that kills host cells by disrupting the transduction of extracellular regulatory signals

Answer 12

Lethal factor

Question 13

EF +LF =

Answer 13

Edema

Question 14

LF+PA

Answer 14

Death

Question 15

Common disease in livestock.

Answer 15

Anthrax

Question 16

How does the anthrax spread?

Answer 16

When animals eat plants that contaminated with spore.

Question 17

T or F
Human are infected primarily as a result of accidental or occupational exposure to animals or animals product.

Answer 17

True

Question 18

Other name of Anthrax disease

Answer 18

Woolsorter’s disease
Ragpicker’s disease

Question 19

3 form of anthrax

Answer 19

Cutaneous anthrax
Inhalation anthrax
Gastrointestinal anthrax

Question 20

It occurs when wounds are contaminated with anthrax spores acquired through skin, cuts, abrasions, insect bites.

Answer 20

Cutaneous anthrax

Question 21

The majority of anthrax cases

Answer 21

Cutaneous anthrax

Question 22

Cutaneous anthrax infection starts with ___.

Answer 22

Small pimple /papule

Question 23

Cutaneous anthrax
A ring of vesicles develops, and the vesicle _______ to form ___.

Answer 23

Coalase
Erythematous ring

Question 24

What do you call the dark area in the center of a ring that cause ulcers and dryness.

Answer 24

Eschar/black eschar

Question 25

Forming depresse black necrotic central called.

Answer 25

Eschar/black eschar

Question 26

Form of anthrax that is known as ___.

Answer 26

Woolsorter's disease

Question 27

Acquired when sporees are inhaled into pulmonary parenchyma

Answer 27

Inhalation anthrax

Question 28

Most deadly type of anthrax.

Answer 28

Inhalation anthrax

Question 29

Bioterrorism agent

Answer 29

Inhalation anthrax

Question 30

Occurs when the spores are inoculated into lesion on the intestinal mucosa after ingestion of spores.

Answer 30

Gastrointestinal anthrax

Question 31

What are the symptoms of GIT anthrax?

Answer 31

Abdominal pain Nausea Anorexia Vomiting Bloody diarrhea

Question 32

It is characterized by soft tissue infection associated with skin popping or direct inject of spores in tissue

Answer 32

Injection anthrax

Question 33

Lab diagnosis Large Squared-ended Gram(+) Rod (single /chain)

Answer 33

Bacillus

Question 34

The chain of bacillus resembles ___.

Answer 34

Bamboo rods.

Question 35

In the gram stain of bacillus, the vegetative cell has a clear zone that surrounds the colony in is known as ___.

Answer 35

Capsule

Question 36

Cultural characteristics of Bacillus anthrax.

Answer 36

Nonhemolytic, large, gray, flat

Question 37

The colonies of B. anthracis is known as ___.

Answer 37

Medusa head

Question 38

T or F The consistency of B. anthracis in agar is Tenacious consistency (beaten egg white).

Answer 38

True

Question 39

Recommend agar for B. anthracis.

Answer 39

PEA agar

Question 40

Which of the following is not the identification of B. anthracis? A. Produce mannitol B. Nonmotile C. Produce lecithinase D. Grow in high salt(7%NaCl and low pH)

Answer 40

A.

Question 41

After how many hours of incubation for the observation of B. anthracis and at what temp?

Answer 41

After 3-6 hours At 37°C

Question 42

Type of Bacillus sp. That is responsible for food poisoning and oppurtunistic infection.

Answer 42

Bacillus cereus

Question 43

Food poisonning of B. cereus in two form.

Answer 43

Diarrheal Emetic form

Question 44

Form of food poisoning caused by B. cereus due to ingestion of meet, poultry, vegetables, pastas.

Answer 44

Diarrheal

Question 45

Diarrheal form -Clin. Indistinguishable from diarrheal cause by ___.

Answer 45

Clostridium perifringens