Other Technologies and Automation

Question 1

Which technology has the following advantages:

• AHG testing can be performed without multiple saline washes
• Check cells are not needed to check for AHG reagent added for negative results

Answer 1

Gel technology

Question 2

Gel technology results are valid for how long?

Answer 2

up to 3 days

Question 3

In gel technology, what is located in the reaction chamber?

Answer 3

reagents (including patient sample when added)

Question 4

In gel technology, where is the gel located?

Answer 4

in the column

Question 5

TRUE OR FALSE: Gel technology provides a more stable endpoint, more reproducible results, and reduces the variability associated with the physical resuspension of RBC buttons.

Answer 5

TRUE

Question 6

ABO forward grouping cards contain gel that contain…

Answer 6

anti-A, anti-B, and anti-A,B

Question 7

Which antisera can help detect subgroups of type A and/or B?

Answer 7

anti-A,B

Question 8

The Rh typing card contains gel that contains

Answer 8

anti-D

Question 9

The Rh phenotyping card contains what in the gel?

Answer 9

anti-D, anti-C, anti-c, anti-E, anti-e, and a control

Question 10

For compatibility testing, antibody detection/ID, the microtubes are filled with gel that contain what?

Answer 10

anti-IgG

Question 11

What kind of gel is used in the gel technology cards?

Answer 11

dextran-acrylamide gel

Question 12

A mixed-field result in gel technology may be seen when?

Answer 12

post transfusion

Question 13

TRUE OR FALSE: Rouleaux may cause a problem when running gel technology.

Answer 13

TRUE

Question 14

What is the difference between 1st and 2nd generation Solid-Phase Red Cell Adherence (SPRCA) tests?

Answer 14

1st generation: target antigen is added by the user

2nd generation: target antigen is bound to microplates by the manufacturer

Question 15

A monolayer of RBC coating the bottom of the microplate is what type of result?

Answer 15

positive

Question 16

Protein A is a cell wall component of which organism?

Answer 16

Staphylococcus aureus

Question 17

Protein A has high affinity to what portion of most Ig?

Answer 17

Fc portion

Question 18

TRUE OR FALSE: HLA antibodies (Class 1 and 2) can be screen for via ELISA methods.

Answer 18

TRUE

Question 19

Which solid-phase method is used to screen and identify platelet antibodies?

Answer 19

Solid-Phase ELISA

Question 20

The solid-phase ELISA test (MACE) is used primarily for

Answer 20

compatibility testing

Question 21

Which solid-phase product provides well-characterized platelet glycoproteins that are either immobilized directly or captured my monoclonal antibodies to wells of a micro well plate?

Answer 21

PAK products

Question 22

Solid-phase PAK products allow for detections and differentiations of…

Answer 22

antibodies that bind to platelet-specific glycoproteins

Question 23

Gel technology can detect which classes of Ig?

Answer 23

IgG and IgM

Question 24

SPRCA can only detect which class of Ig?

Answer 24

IgG

Question 25

Do SPRCA or gel technology detect complement?

Answer 25

no

Question 26

Which method requires washing, solid-phase and/or gel technology?

Answer 26

solid-phase

Question 27

Which technology utilizes both protein A and protein G?

Answer 27

Affinity column

Question 28

Which technology utilized a beaded gel matrix?

Answer 28

Affinity column

Question 29

Which protein binds to the Fc portion of all IgG subclasses?

Answer 29

Protein G

Question 30

Which protein binds to the Fc portion of all IgG subclasses, except IgG3

Answer 30

Protein A