Pipetting Techniques

Question 1

for the quantitative transfer of reagents and the preparations of serial dilutions of specimens
such as serum.

Answer 1

Pipettes

Question 2

Can make the specimen unacceptable

Answer 2

Lipemia, hemolysis, and bacterial contamination

Question 3

pressure of excess bilirubin in the blood system, turbid serum

Answer 3

Icteric

Question 4

Blood specimens should be collected ____ to avoid chyle

Answer 4

Before a meal

Question 5

a milky bodily fluid consisting of lymph and emulsified fats, or free acids

Answer 5

Chyle

Question 6

dark to very dark yellow, brownish like sample

Answer 6

Icteric Sample

Question 7

Expect patients with steric sample to have a very high ___ or ____

Answer 7

bilirubin or ALT/SGPT

Question 8

ALT normal value parameter for liver diseases

Answer 8

55 lU/L

Question 9

Fatty liver disease

Answer 9

> 55 lU/L

Question 10

• damaged /scarred liver

Answer 10

> 200 |U/L

Question 11

liver cirrhosis

Answer 11

> 1000 IU / L

Question 12

liver cirrhosis reversible stage

Answer 12

Stage 1-3

Question 13

liver cirrhosis irreversible stage

Answer 13

stage 4

Question 14

Turbid to milky consistency of the sera

Answer 14

Lipemic Sample

Question 15

Expect patients with Lipemic Sample to have a very high ___ and ____

Answer 15

Cholesterol and Triglycerides

Question 16

Calculation for total cholesterol

Answer 16

HDL + LDL + Triglycerides / 5

Question 17

Red sample (Destruction of red blood cells)

Answer 17

Hemolyzed Sample

Question 18

hemolyzed sample means elevated level of ___

Answer 18

Elevated levels of Potassium

Question 19

Potassium Nv

Answer 19

NV: 3.5 - 5.0 mmol / L

Question 20

Number of samples per hour for manual pipetting

Answer 20

5-10 samples per hour

Question 21

the curvature in the top surface of a liquid

Answer 21

Meniscus

Question 22

The greatest potential hazard is when ___ pipetting is done instead of mechanical suction.

Answer 22

mouth pipetting

Question 23

offers a way for labs to incrementally scale up
production and increase reproducibility

Answer 23

Semi-Automatic Pipettes

Question 24

Number of samples per hour for Semi-Automatic Pipettes

Answer 24

11-100 samples per hour

Question 25

most valuable in high-throughput applications that benefit from completely removing human movements

Answer 25

Automation

Question 26

can process hundreds of samples at a time and follow highly complex methods without deviation

Answer 26

Automation

Question 27

can cause problems with the. measurement and delivery of samples and solutions.

Answer 27

Bubbles and viscous solutions

Question 28

Bubbles =

Answer 28

wrong procedure handling

Question 29

Complement can be inactivated by heating to ___ C for ___minutes, or after ___hours, reinactivared by heating for __ minutes.

Answer 29

heating to 56 C for 30 minutes, or after 4 hours, reinactivared by heating for 10 minutes.

Question 30

disposable plastic pipes used to transfer small volumes of liquids

Answer 30

Transfer pipette

Question 31

"To deliver" (TD) types that have the bulb closer to the center and accurately deliver a fixed volume of aqueous solution

Answer 31

Volumetric pipette

Question 32

TD types that have the bulb closer to the delivery tip because they deliver viscous fluids.

Answer 32

Ostwald Folin pipette

Question 33

These pipettes deliver an accurate volume by being "blown out" using a pipetting bulb.

Answer 33

Ostwald Folin pipette

Question 34

a pipette with its volume, in increments, marked along the tube.

Answer 34

Graduated pipette

Question 35

It is used to accurately measure and transfer a volume of liquid from one container to another.

Answer 35

Graduated pipette

Question 36

The orifice, or tip opening, is larger than in other pipettes.

Answer 36

Serological pipette

Question 37

The rate of fall of liquid is much too fast for great accuracy or precision.

Answer 37

Serological pipette

Question 38

Serological pipette is calibrated to the tip and must be ___ to deliver the entire volume.

Answer 38

Blown out

Question 39

The need to blow out is indicated by the ___ at the top of the pipette

Answer 39

etched rings

Question 40

calibrated between marks cannot be "blown out"

Answer 40

mohr pipette

Question 41

allow rapid repetitive measurement and delivery of predetermined volumes of reagents and specimens

Answer 41

Micropipette

Question 42

piston-operated devices that allow repeated, accurate, reproducible delivery of specimens, reagents, and other liquids requiring measurement in small amounts

Answer 42

Micropipette

Question 43

Difference between mohr and serological pipette

Answer 43

mohr- graduated between two marks Serological - grade mark down to the tip

Question 44

Common Pipetting Errors

Answer 44

1. Loose pipette tip 2. Tilting the pipettor 3. Plunger quick release (will cause air bubbles) 4. Second stop draw