POC: Drug testing

Question 1

When are urine drug screening tests used

Answer 1

sports
workplace
homes
law enforcement
pain clinics

Question 2

T or F: urine drug tests use lateral flow tech

Answer 2

T- not same as pregnancy tests though
— uses competitive binding instead bw Ab + drug
— still has Ab + gold — detection but if drug present —-bind better to detector than Ab+gold—- no formation of line

+ result—— no line present

Question 3

What indicates drug is present

Answer 3

NO LINE
- if drug present — kicks AB+ gold off the capture molecule — no line

Question 4

T or F: if get + result in urine drug screen , you can confirm that they had the drug in the system for sure

Answer 4

F - need to confirm with analytical methods (GC + MS)
——- urine drug screeens have a high risk of false + (low specificity)

Question 5

Why do we use a competition assay to detect drugs instead of sandwich method that pregnancy tests use

Answer 5

allows us to screen for multiple drugs at once + drugs —- are smaller + decreased MW (easier to bind + detect this way)

Question 6

T or F: urine drug screens normally measures drug metabolites

Answer 6

T

Question 7

How is the urine sample collected for urine drug screens

Answer 7

they pee direclty into the cup/test

Question 8

What can cause false -

Answer 8

timing of sample collection off ( took drug too long ago or too recently)
drug taken not detected by test
urine sample messed with (bleach or other shit added)
sample diluted (drug levels too low — don’t reach cutoff of detection )

Question 9

T or F: urine drug screens normally measures drug metabolites

Answer 9

T

Question 10

How is the urine sample collected for urine drug screens

Answer 10

they pee direclty into the cup/test

Question 11

What can cause false +

Answer 11

cross-reactivity with other drugs or food that also bind to capture molecule (kick off Ab — no line seen)
- ex// doxylamine — false + for methadone

Question 12

What are methods to ensure urine sample not tampered with

Answer 12

1) tests to look for prescence of oxidants: bleach, perodixes (interfere with test + may destroy drug if present in pee)

2) Test for specific gravity to ensure not diluted

3) test pH:

4) Temp check: is the pee old?

Question 13

What adulterants did our lab urine drug test test for

Answer 13

oxidants, SG, and pH

Question 14

Instructions for use

Answer 14

pee into cup (30-90mL); peel off blue label to see results
- read at 5 minutes (up to 60 mins)