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CC 2 LAB > Prelims Agappe > Flashcards

Prelims Agappe Flashcards

1
Q

AMYLASE (AMS)

A

EC 3.2.1.1

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2
Q

LIPASE (LPS)

A

EC 3.1.1.3

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3
Q

CREATINE KINASE (CK)

A

EC 2.7.3.2

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4
Q

LACTATE DEHYDROGENASE (LDH)

A

EC 1.1.1.27

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5
Q

ASPARTATE AMINOTRANSFERASE (AST)

A

E.C. 2.6.1.1

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6
Q

ALANINE AMINOTRANSFERASE (ALT)

A

E.C. 2.6.1.2

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7
Q

ALKALINE PHOSPHATASE (ALP)

A

E.C. 3.1.3.1

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8
Q

ACID PHOSPHATASE (ACP)

A

E.C. 3.1.3.2

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9
Q

Activators of AMY

A

Calcium
Chloride

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10
Q

Lipase activators

A

Colipase
Bile salts

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11
Q

Aspartate and Alanine activators

A

Pyridoxal phosphate

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12
Q

Alkaline activators

A

Optimal pH: 9.0-10.0 Activator: Mg 2 + & Zinc

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13
Q

Acid activators

A

Optimal pH: 5.0 Activator = Mg 2 + & Zinc

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14
Q
  1. Amyloclastic method
  2. Saccharogenic method
  3. Chromogenic method
  4. Continuous monitoring method
A

Amylase

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15
Q
  1. Classic Cherry-Crandall Method (olive oil)
  2. Turbidimetric Methods
  3. Colorimetric Methods
A

Lipase

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16
Q

Electrophoresis
Immunoinhibition
Immunoassay
Ion-exchange chromatography

A

CK isoenzyme

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17
Q

Tanzer Gilvarg
Oliver Rosalki

A

CK enzyme

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18
Q

Wacker
Wrobleuski

A

Ldh

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19
Q

Karmen Method - Uses a coupled enzymatic reaction

Indicator: Malate Dehydrogenase

Monitors absorbance change at_____

  • Measures NADH oxidation to NAD+
  • Optimal pH Range:_____
A

Aspartate

340 nm

7.3 to 7.8

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20
Q

Coupled Enzymatic Reaction:

  • Utilizes Lactate Dehydrogenase (LD) as the indicator enzyme
  • LD catalyzes reduction of pyruvate to lactate
  • Simultaneous oxidation of NADH to NAD+
A

Alanine Aminotransferase

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21
Q

Reitman & Frankel

  • Colorimetric/Endpoint
  • Color developer:______
  • Color intensifier:_____
A

2,4 Dinitrophenylhydrazine (DNPH)

0.4N Sodium hydroxide (NaOH)

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22
Q

Reitman & Frankel

  • Colorimetric/Endpoint
A

Alanine Aminotransferase

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23
Q

Continuous-Monitoring Technique:

Based on Bowers and McComb method

24
Q

ALKALINE

Continuous-Monitoring Technique: Based on Bowers and McComb method

Most specific method Calculates ALP activity using molar absorptivity of p-nitrophenol

Substrate =_______

Positive result =______

A

para-Nitrophenylphosphate (PNPP); colorless

Yellow (p-nitrophenol)

25
Roy and Hillman (Quantitative Endpoint) Substrate:
Thymolphthalein monophosphate ACID
26
Babson, Read & Phillips (Continuous Monitoring) Substrate:
Alpha-naphthyl phosphate ACID
27
Immunochemical Techniques: - Radioimmunoassay (RIA) -Counterimmunoelectrophoresis - Immunoprecipitation - Immunoenzymatic Assay (Tandem E)
ACID
28
Amylase RS
1000 25
29
CK RS
1000 40
30
LDH RS
1000 10
31
AST ALT RS
1000 100
32
ALP RS
1000 20
33
ACP
1000 100
34
CK NR
SERUM: 25 - 86 U/L URINE: < 470 U/L
35
LIPASE NR
60 U/L
36
CK NR
Serum up to : 24 U/L % CK- MB :6-25 %
37
LDH NR 1- 3 years :
490-730 U/L
38
LDH NR 4 - 9 years :
320-520 U/L
39
LDH NR 10 - 13 years :
250-500 U/L
40
LDH NR Adults :
225-450 U/L
41
LDH NR
1- 3 years :490-730 U/L 4 - 9 years :320-520 U/L 10 - 13 years :250-500 U/L Adults :225-450 U/L
42
Aspartate NR
Serum up to 46 UL
43
Alanine NR
Serum up to 49 U/L
44
Alkaline NR Women
35 - 104 U/L
45
Alkaline NR Men
40 - 129 U/L
46
Alkaline NR Children up to15 yrs:
54 - 369 U/L
47
Alkaline NR
Women 35 - 104 U/L Men 40 - 129 U/L Children up to15 yrs: 54 - 369 U/L
48
ACP NR Men
Men up to (U/I) 3.6; 5.0; 6.5
49
ACP NR women
Women up to (U/I) 3.0; 4.2; 5.5
50
Amylase factor
3178
51
CK factor
8254
52
LDH factor
16030
53
AST factor
1745
54
Alanine factor
1745
55
Alkaline factor
2757
56
Acid factor
25°C = 149 30°C or 37°C = 248

CC 2 LAB (35 decks)

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