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Mycology PPT > PRIMARY RECOVERY MEDIA AND DIFFERENTIAL MEDIA > Flashcards

PRIMARY RECOVERY MEDIA AND DIFFERENTIAL MEDIA Flashcards

(68 cards)

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1
Q

Primary recovery of saprobic and pathogenic fungi

A

Brain-heart infusion agar

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2
Q

Brain-heart infusion agar composition

A

Brain-heart infusion, enzymatic digest of animal tissue, enzymatic digest of casein, dextrose, sodium chloride

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3
Q

Brain-heart infusion agar with antibiotics composition

A

Brain-heart infusion, enzymatic digest of animal tissue, enzymatic digest of casein, dextrose, sodium chloride, antibiotics

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4
Q

Recovery of fungi from blood

A

Brain-heart infusion biphasic blood culture bottles

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5
Q

Brain-heart infusion biphasic blood culture bottles composition

A

Brain-heart infusion, peptone, glucose, disodium phosphate

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6
Q

Isolation and presumptive identification of yeast and filamentous fungi

A

Chromogenic agar

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7
Q

Chromogenic agar composition

A

Chromopeptone Glucose Chromogen mix Chloramphenicol

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8
Q

Primary recovery of dermatophytes; recommended as screening medium

A

Dermatophyte test medium

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9
Q

Dermatophyte test medium composition

A

Dextrose, cycloheximide, gentamycin, chloramphenicol, phenol red

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10
Q

Primary recovery of pathogenic fungi exclusive of dermatophytes

A

Inhibitory mold agar

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11
Q

Inhibitory mold agar composition

A

Chloramphenicol, casein, dextrose, starch, sodium phosphate, magnesium sulfate, sodium chloride, manganese sulfate

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12
Q

Potato flake agar composition

A

Potato flakes, glucose, cycloheximide, chloramphenicol, bromthymol blue

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13
Q

Primary recovery of dermatophytes

A

Mycosel agar

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14
Q

Mycosel agar composition

A

Cycloheximide, chloramphenicol, dextrose

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15
Q

Primary recovery of saprobic and pathogenic fungi

A

Sabouraud Dextrose with Brain Heart Infusion (SABHI) agar

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16
Q

Sabouraud Dextrose with Brain Heart Infusion (SABHI) agar composition

A

Sabouraud dextrose, brain-heart infusion agar

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17
Q

Primary recovery of pathogenic fungi exclusive of dermatophytes

A

Yeast-extract phosphate agar

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18
Q

Yeast-extract phosphate agar composition

A

Yeast extract, dipotassium phosphate, chloramphenicol

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19
Q

Detection of ascospores in ascosporogenous yeasts

A

Acetate Ascospore agar

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20
Q

Acetate Ascospore agar composition

A

Potassium acetate, yeast extract, dextrose

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21
Q

Identification of
Cryptococcus, Trichosporon, and Rhodotorula spp

A

Christensen’s urea agar

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22
Q

Christensen’s urea agar composition

A

2% urea, phenol red

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23
Q

Identification of Candida albicans by chlamydospore production Identification of C. albicans by microscopic morphology

A

Cornmeal agar with Tween 80 and trypan blue

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24
Q

Cornmeal agar with Tween 80 and trypan blue composition

A

Cornmeal, Tween 80, trypan blue

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25
Conversion of the dimorphic fungus Blastomyces spp. from mold to yeast form
Cottonseed conversion agar
26
Cottonseed conversion agar composition
Cottonseed meal, glucose
27
Differential identification of Aspergillus spp.
Czapek’s agar
28
Czapek’s agar composition
Sodium nitrate, sucrose, yeast extract
29
Identification of Cryptococcus neoformans and Cryptococcus gattii
Niger seed agar (birdseed agar)
30
Niger seed agar (birdseed agar) composition
Guizotia abyssinica seed, dextrose, chloramphenicol
31
Detection of nitrate reduction to confirm Cryptococcus spp.
Nitrate reduction medium
32
Nitrate reduction medium composition
Potassium nitrate, peptone, meal extract, sulfanilic acid, N, N-dimethyl-1-naphthylamine
33
Demonstration of pigment production by Trichophyton rubrum Preparation of microslide cultures and sporulation of dermatophytes
Potato dextrose agar
34
Potato dextrose agar composition
Potato infusion, D(+) glucose
35
Identification of Microsporum audouinii
Rice medium
36
Rice medium composition
White rice extract, polysorbate 80
37
Identification of Trichophyton spp.
Trichophyton agars 1-7
38
Trichophyton agars 1-7 composition
Casamino acids, dextrose, monopotassium phosphate, magnesium sulfate, amino acids, ammonium nitrate
39
Detection of Cryptococcus spp. Differentiate Trichophyton mentagrophytes from Trichophyton rubrum Detection of Trichosporon spp,
Urea agar
40
Urea agar composition
Peptone, dextrose, sodium chloride, monopotassium phosphate, urea, phenol red
41
Identification of yeasts by determining fermentation
Yeast fermentation broth
42
Yeast fermentation broth composition
Yeast extract, peptone, bromcresol purple, and a specific carbohydrate
43
Identification of yeasts by determining carbohydrate assimilation
Yeast nitrogen-base agar
44
Yeast nitrogen-base agar composition
Ammonium sulfate, carbon source
45
Primary recovery media
Brain-heart infusion agar Brain-heart infusion agar with antibiotics Brain-heart infusion biphasic blood culture bottles Chromogenic agar Dermatophyte test medium Inhibitory mold agar Potato flake agar Mycosel agar Sabouraud dextrose woth brain heart infusion agar Yeast-extract phosphate agar
46
Primary recovery of saprobic and pathogenic fungi
BHI agar Potato flake agar Sabouraud Dextrose with Brain Heart Infusion (SABHI) agar
47
Primary recovery of pathogenic fungi exclusive of dermatophytes
Yeast-extract phosphate agar Inhibitory mold agar Brain-heart infusion agar with antibiotics
48
Primary recovery of dermatophytes
Dermatophyte test medium Mycosel agar
49
DIFFERENTIAL TEST MEDIA
Acetate Ascospore agar Christensen’s urea agar Cornmeal agar with Tween 80 and trypan blue Cottonseed conversion agar Czapek’s agar Niger seed agar (birdseed agar) Nitrate reduction medium Potato dextrose agar Trichophyton agars 1-Urea agar Yeast fermentation broth Yeast nitrogen-base agar
50
The agar provides a rich medium for bacteria, yeast, and pathogenic fungi.
Brain-heart infusion agar
51
The agar provides a rich medium for yeast and pathogenic fungi, including systemic dimorphic fungi.
Brain-heart infusion agar (fungal formulation) with antibiotics
52
Chromogen mix contains substrates that react with enzymes produced by differ- ent organisms that result in the production of character- istic color changes
Chromogenic agar
53
Dermatophytes produce alkaline metabolites, which raise the pH and change the medium from red to yellow.
Dermatophyte test medium
54
Examine plates for growth. Chloramphenicol inhibits bacterial growth.
Inhibitory mold agar
55
Growth is enhanced by a pH alkaline reaction of fungus. Chloramphenicol and anti- biotics inhibit the growth of bacteria and nonpathogenic fungi.
Potato flake agar
56
Inhibits bacteria and saprophytic fung
Mycobiotic or mycosel agar
57
Isolates and enhances growth of all fungi including the yeast phase of dimorphic fungi.
Sabouraud dextrose with brain-heart infusion (SABHI) agar
58
Enhances the recovery and sporulation of Blastomyces and Histoplasma capsu- latum from contaminated specimens
Yeast-extract phosphate agar with ammonia
59
Potassium acetate is neces- sary, and yeast extract increases the sporulation of yeasts.
Acetate Ascospore agar
60
Produces urease and a change in the pH.
Christensen’s urea agar
61
enhances the production of chlamydospores, pseudohy- phal and arthrospore forma- tion. The addition of trypan blue provides a contrasting background for observing the morphologic features of yeasts.
Cornmeal agar with Tween 80 and trypan blue
62
Produces characteristic fea- tures of yeast and fungus of any organism that can use sodium nitrate.
Czapek’s agar
63
C. neoformans and C. gattii produce the enzyme phenol oxidase, resulting in a brown pigment through metabolism of caffeic acid
Niger seed agar (birdseed agar)
64
Creatinine enhances the mela- nization of some strains of C. neoformans
Niger seed agar (birdseed agar)
65
Carbohydrate and potato infu- sion promotes the growth of yeasts and molds, and the low pH (tartaric acid) partially inhibits bacterial growth.
Potato dextrose agar
66
may be dif- ferentiated by growth in the presence of different amino acids.
Trichophyton agars 1–7
67
Most yeasts produce acid, which is indicated by a change in the solution from purple to yellow as a positive fermenter.
Yeast fermentation broth
68
Assimilation of carbon by yeast cells produces a positive result
Yeast nitrogen-base agar

Mycology PPT (17 decks)

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