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Biol 3400 > Protein trafficking between membranes. > Flashcards

Protein trafficking between membranes. Flashcards

1
Q

What is the coat used for?

A

Budding and scission of vesicles.

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2
Q

What happens to the coat before tethering occurs?

A

It dissociates from the vesicle.

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3
Q

What member of the endomembrane is responsible for endocytosis?

A

Lysosomes

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4
Q

What member of the endomembrane is responsible for Excocytosis?

A

Endoplasmic reticulum and Golgi aparatus

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5
Q

What is the sequence of compartments proteins go through to leave a cell

A

Golgi
Vesicle
Membrane

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6
Q

What are the two processes for Exocytosis

A

Constitutive and regulated

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7
Q

What are common neurofiliments?

A

NF-L, NF-M, NF-H

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8
Q

Where are neurofiliment proteins found?

A

MTOC in neuronal cells.

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9
Q

What do Neurofilaments do?

A

Stabilize the organization of the endoplasmic reticulum and golgi complex

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10
Q

What is the regulator mechanism for endocytosis

A

Enzymes are released only when signalled by appropriate stimuli

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11
Q

Where do vesicles move in retrograde transport

A

From golgi to ER

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12
Q

How are vesicles exiting the endoplasmic reticulum formed?

A

COP II
Sorting proteins in cytoplasmic tail.
Soluble proteins eneter bulkflow or bind to signal proteins.

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13
Q

What is the targeting signal used to retain resident Golgi protein

A

Signal anchor and flank sequence.

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14
Q

What are SNARES used for

A

Docking

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15
Q

Where do COPI vesicles move proteins in forward transport?

A

From CGN to TGN (Cis golgi to trans golgi)

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16
Q

Where do COPI vesicles move proteins in reverse transport?

A

From VTC or Golgi to ER.

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17
Q

What is needed for COPI to do reverse transport?

A

KDEL sequence.

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18
Q

What are the ordered Steps prior to fusion of a vesicle

A 
Budding
Scission 
Uncoating
Tethering
Docking
Fusion
19
Q

What type of vesicle transport from Golgi to ER in retrograde transport

A

COPI

20
Q

What would a cell lacking functional T-SNAREs look like

A

Docking wouldn’t happen

21
Q

What is the complex that unwinds v/t-SNARES

A

NSF

22
Q

What is the role of SNARE proteins?

A

To allow for fusion by docking.

23
Q

Where does ATP hydrolysis occur in the process of fusion to a target membrane?

A

In unraveling snares via NSF

24
Q

What does Hsc70 do in endocytosis

A

Uncoats

25
Q

What is Hsc70

A

ATPase

26
Q

What structure does clathrin form around vesicles that originate in the plasma membrane

A

A “cage”

27
Q

What is the function of adaptin proteins

A

to link clathrin to ligands

28
Q

How do adaptor proteins link clathrin to membrane proteins?

A

Through ligands.

29
Q

What is the retention signal for resident ER proteins

A

KDEL

30
Q

What would happen if a cell failed to place mannose-6-phosphate on a protein?

A

Soluble lysosomal enzymes wouldn’t get sorted.

31
Q

List the differences between early endosome and lysosome

A

Lysosome are more acidic and have degradive enzymes.

32
Q

What is the order of endosomes

A

Early endosome to late endosome to lysososme

33
Q

How are clathrin vesicles used in endocytosis

A

They bring material across the plasma membrane and deliver it to endosomes.

34
Q

Where do clathrin bring proteins when coming from the golgi in endocytosis

A

to late endosomes

35
Q

Where do clathrin bring proteins when coming from the Plasma membrane in endocytosis

A

Early endosomes.

36
Q

Where do clathrin bring protein in exocytosis

A

From condensing vacuole to Golgi

37
Q

What is the role of clathin in fusion

A

They are a transport vesicle. they come off before fussion.

38
Q

What are coated pits made out of?

A

COPI COPII and Clathrin

39
Q

What is the role of RabGTP

A

Tethering.

40
Q

What is the exit side of the Golgi Network

A

Trans (TGN)

41
Q

What is the entrance side of the Golgi Network

A

Cis (CGN)

42
Q

How does KDEL incite retrival?

A

There is a ligand on the KDEL that attaches to COPI and moves to CGN

43
Q

What degree is the sturcture of Clathrin

A

Quatrenary

44
Q

What is Clathrin triskelion made of?

A

Three heavy chains, three light chains.

Biol 3400 (9 decks)

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