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Flashcards in Proteins Deck (19):
1

Enzyme

Biological catalysts that increase reaction rate and control biological processed

2

How can proteins be classified on basis of shape

Fibrous (elongated and insoluble) further classified as collagens (connective tissue), elastins (elastic tissue) and keratins (horn/nail/wool/hair). Globular (oval and soluble)

3

Name the ends of a protein chain

N-terminus is the end with the amino group C-terminus is the end with the carboxyl group

4

Isoelectric point

The pH at which the charge of a protein is 0

5

Electrophoresis

a method of seperating proteins based on charge by seperating them through agar gel in an electric field

6

Ion exchange chromatography

a method of seperating proteins based on charge where as pH is changed proteins with only a specific charge can pass through a charged bead matrix

7

Gel Filtration

passing proteins through a gel matrix where proteins of different sizes move through at different rates

8

Isoelectric point ppt

when the pH of the solution is at the proteins isoelectric point the protein looses it's charge and becomes its least soluble and may precipitate

9

(NH4)2SO4 ppt

Specific concentrations of (NH4)2SO4 precipitate specific proteins by taking place of water molecules around the protein that allows it dissolve.

10

Primary structure

sequence of amino acids

11

Edmand Degredation Technique

A method of sequencing proteins where amino acids are removed one by one from the N-terminus by using the edmans reagent followed by trifluoroacetic acid.

12

Secondary structure

alpha helix and beta sheet

13

Tertiary structure

Complex folding of the overall protein

14

Quaternary structure

multiple polypeptides coming together to form a protein

15

Denature

Protein loosing structure or function by organic solvent (H bonds), detergents (Hydrophobic bonds), pH change (electrostatic bonds) and heat (non-covalent bonds)

16

Dimer

Proteins made of 2 polypeptides

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Trimer

protein with 3 polypeptides

18

Homodimer

Proteins containing 2 identical polypeptides

19

Heterodimer

protein made of 2 different polypeptides