Research/tests Flashcards
(5 cards)
In a paired t-test, what p value shows a statistically significant difference between the samples?
p < 0.05 (5%)
In a paired t-test what does the p value represent?
The probability that the difference seen between the 2 groups has occurred by chance
Sensitivity vs specificity
Sensitivity - how well a test identifies those with the disease (true positive)
Specificity - how well a test identities those without the disease (true negative)
How to calculate sensitivity
How to calculate specificity
Sensitivity = true positive / (true positive + false negative)
Specificity = true negative / (true negative + false positive)
Whole genome seq vs karyotype vs FISH vs DNA methylation tests
WGS:
comprehensive, base-pair level analysis, especially undiagnosed rare diseases
Karyotyping:
suspected gross abnormality e.g. whole-chromosome/large structural such as trisomy/translocation
FISH:
targeting specific known genetic abnormality, more rapid
DNA methylation:
Prader-Willi/Angelman syndrome - abnormal methylation plays key role