Research/tests Flashcards
(8 cards)
In a paired t-test, what p value shows a statistically significant difference between the samples?
p < 0.05 (5%)
In a paired t-test what does the p value represent?
The probability that the difference seen between the 2 groups has occurred by chance
Sensitivity vs specificity
Sensitivity - how well a test identifies those with the disease (true positive)
Specificity - how well a test identities those without the disease (true negative)
How to calculate sensitivity
How to calculate specificity
Sensitivity = true positive / (true positive + false negative)
Specificity = true negative / (true negative + false positive)
Whole genome seq vs karyotype vs FISH vs DNA methylation tests
WGS:
comprehensive, base-pair level analysis, especially undiagnosed rare diseases
Karyotyping:
suspected gross abnormality e.g. whole-chromosome/large structural such as trisomy/translocation
FISH:
targeting specific known genetic abnormality, more rapid
DNA methylation:
Prader-Willi/Angelman syndrome - abnormal methylation plays key role
RCT vs case control study
RCT
- prospective
- participants randomised to either receive experimental treatment/control treatment i.e. placebo
- outcomes compared between the 2 groups
- reduces bias as random
Case control
- usually retrospective
- outcomes after treatment compared with outcomes in suitably matched controls
- no randomisation so can be bias
Risk measure in cohort studies
Relative risk
Prospective
Follow groups based on exposure to see what diseases develop e.g. non smoker vs smoker lung ca risk
Exposure -> disease
Risk measure in case control studies
Odds ratio
Retrospective
Look at people with and without a disease to find prior exposures e.g. lung ca vs healthy controls, looking at smoking exposure
Disease -> exposure