Rh system - C and E antigens

Question 1

Rh null (regulator)

Answer 1

absence of RhAg regulator - no Rh antigens on the cell

Question 2

Rh null (amorph)

Answer 2

Rh genes genetically mutated to missense genes

• RhAg on cells w/o Rh antigens
• RhCE mutated on RhD

Question 3

describe insiude the cell Rh null

Answer 3

low water, low cation = overactive ATPase

Question 4

serology of Rh null

Answer 4

lack Lw and FY5
reduced SsU (glycophorin B)

Question 5

true or false
C and c are antithetical

Answer 5

true
- 4 amino acid difference but differ in expression

Question 6

impact of abnormal alleles on C antigen

Answer 6

leads to mutations
- anti C with C pos

Question 7

Cw and Cx

Answer 7

C antigen mutation in caucasians
- weak C expression

Question 8

RHDIII-CE hybrid

Answer 8

in 25% of africans
- no D antigen and altered C antigen

Question 9

% expression big and little C - white

Answer 9

C:68%
c: 80%

Question 10

% expression big and little C - black

Answer 10

C: 20%
c: 96%

Question 11

% expression big and little C - chinese

Answer 11

C: 90%
c: 60%

Question 12

% expression big and little C - indian

Answer 12

C: 85%
c: 50%

Question 13

% expression big and little C - hispanic

Answer 13

C: 70%
c: 40%

Question 14

immunogenicity of Rh system

Answer 14

D>c>E>C>e

Question 15

assumption of little e

Answer 15

high incidence - most people will have little e

Question 16

E/e antibody

Answer 16

IgG, reacts in coombs phase
- dosage
- enhanced by enxymes
- not compliment binding

Question 17

what is unique about anti-E

Answer 17

can be weak naturally occuring

Question 18

describe Fischer-Race nomenclature

Answer 18

3 individual genes for 3 antigens
- DCcEe naming

Question 19

describe Weiner nomenclature

Answer 19

one Rh gene makes 3 allele antigens
- Rh naming

Question 20

describe Tippette nomenclature

Answer 20

one RhD gene and one RhCE gene on same chromosome one

Question 21

which of the 3 naming systems is correct

Answer 21

tippett

Question 22

describe capellini effect

Answer 22

C in trans blocks D expression
- big C and D on haplotype/heterozygous

Question 23

anti-F

Answer 23

attacks little c little e on same haplotype (compound) -> anti-ce

Question 24

anti-G

Answer 24

D or C
- detected for rhogam

Question 25

concomitant antibodies

Answer 25

found together frequently based on pop frequency

Question 26

D deletion

Answer 26

represented by dashes

Question 27

how does deleted CcEe antigens impact D expression

Answer 27

increases expression (enhances) -> exalted D

Question 28

list 2 causes of D-- (D deletion)

Answer 28

- RhD disrupts RhCE by transcribing over it - RhCE silencer - hispanic

Question 29

most common antigen expression - white

Answer 29

DCe (R1) - 40%

Question 30

most common antigen expression - black

Answer 30

Dce (R0) - 40%

Question 31

most common antigen expression - asian

Answer 31

DCe (R1) - 70%

Question 32

second most common antigen expression - white

Answer 32

dce (r) - 37%

Question 33

second most common antigen expression - black

Answer 33

dce (r) - 25%

Question 34

second most common antigen expression - asian

Answer 34

DcE (R2) - 20%

Question 35

D antigen frequency

Answer 35

85 - lower in white

Question 36

big C antigen frequency

Answer 36

70 - lower in black

Question 37

little c antigen frequency

Answer 37

80 - lower in asian

Question 38

big E frequency

Answer 38

30

Question 39

little e frequency

Answer 39

98

Question 40

Rh system frequencies simplified

Answer 40

D: 80 C:70 c: 80 E: 30 e:98

Question 41

antigen Vs

Answer 41

e antigen mutation Hrs/hrb

Question 42

which antibody reacts with rhesus-baby cells but no rhesus-adult cells - antiD - antiC -atniDu - antiLw

Answer 42

anti LW - higher incidence at birth and decreases with age

Question 43

which population is the weak D test performed in - baby - elderly - cardiac - prenatal

Answer 43

baby