RP1: Enzymes

Question 1

How is a control set up in this practical?

Answer 1

removing enzyme/replacing enzyme with a boiled, denatured enzyme

Question 2

What factors impact enzyme activity?

Answer 2

• pH
• temperature
• concentration

Question 3

Outline how you would set up a practical investigating the rate of reaction using trypsin and milk…

Answer 3

• use a water bath, immerse separate test tubes of specified volume
• mix contents and start timer
• record time taken for sample to turn colourless
• 5 temps, with 3 repeats at each

Question 4

Why do you have to leave the contents in the water bath for 5 minutes before mixing them together?

Answer 4

allow time for temperature of the solutions to equilibrate

Question 5

What precautions should be taken when working with enzymes?

Answer 5

• avoid contact with skin
• wear safety goggles