RP8: DCPIP Flashcards
1
Q
What is the function of the enzyme dehydrogenase in chloroplasts?
A
catalyses the acceptance of electrons by NADP in LDR
2
Q
What is DCPIP?
A
- a redox indicator dye, alternate electron acceptor instead of NADP
- turns blue to colourless when reduced
3
Q
Why is the plant extract chilled?
A
to lower enzyme activity that could break down/hydrolyse chloroplasts
4
Q
How is the control set up?
A
distilled water and chloroplast extract
5
Q
How is light intensity controlled?
A
- measuring distance of lamp
- darker rom, so only light source is the lamp
6
Q
What colour does DCPIP change to when it accepts electrons?
A
blue to colourless
7
Q
What colour standard is used so the end point can be identified?
A
Chloroplast solution only
8
Q
What is the control experiment if you were investing the effect of light intensity on the LDR?
A
no light/ wrap solution in foil
