Flashcards in Serology Deck (10):
What are agglutination tests?
Used to identify antigens or specific antibodies in body fluid or secretion
Discuss the principle for agglutination tests?
1. Antibodies bind to antigens on solid particles in fluid medium.
2. Antibodies cross-link the particles to form a visible clump.
Differentiate Forward from Reverse hemagglutination.
Forward: Known test reagents (anti-A,B, D) added to RBCs with unknown antigens.
Reverse: Add patient's serum to lab RBC's; Test looks for antibodies.
How do you detect Salmonella?
Direct agglutination; If patient serum has antibodies against salmonella, clumping will occur.
How do you detect autoreactive antibodies?
Indirect agglutination: When the antigens or antibodies are artificially attached to the particles. Causes agglutination.
Discuss agglutination inhibition.
Testing the ability to disrupt antigen/antibody interactions. Used for rubella and measles;
1. Mix serum with virus
2. Add RBCs
3. Look for agglutination. NO aggultination is a positive test.
What is a western blot?
An immunoassay used to identify antigens present in low concentrations.
Discuss Western blot testing.
1. Electrophoresis is used to separate proteins
2. Antigens are transferred to nitrocellulose
3. Antibodies are used to detect antigens on nitrocellulose.
What is an elisa immunoassay?
Identifies specific antigens or antibodies in bodily fluid or secretion.