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Immunology & Serology > Serology techniques > Flashcards

Serology techniques Flashcards

1
Q

Clumping & sedimentation of particulate

A

Agglutination

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2
Q

Insoluble particle reacts with Antibody

A

Direct agglutination

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3
Q

(True or False):

Direct non-immune reaction is due to Antigen-Antibody rection

A

False (direct immune)

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4
Q

Latex agglutination test for Rheumatoid factor is an example of what type of agglutination?

A

Indirect / Passive

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5
Q

Antibody is attached to a carrier particle

A

Reverse passive agglutination

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6
Q

Agglutination inhibition is based on competition between ____ & ____

A

Particulate

Soluble antigens

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7
Q

It detects the presence of non-agglutinating antibodies by adding a second antibody

A

Antiglobulin test

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8
Q

Most frequently used bacteria in coagglutination

A

S. aureus

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9
Q

Immunoassay that uses gold as inert particle

A

Sol particle

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10
Q

Immunoassay that uses dye as inert particle

A

Disperse dye

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11
Q

It uses latex particles

A

IMPACT (Immunoassay by particle counting)

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12
Q

It is the combination of soluble antigen w/ antibody forming an insoluble complex

A

Precipiation

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13
Q

(True or False):

Avidity is the strength of primary interaction between single antigenic determinant and Ab binding site

A

False (Affinity)

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14
Q

It is the sum of all attractive forces between Ag-Ab

A

Avidity

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15
Q

Ag and Ab diffuse radially

A

Double dimension

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16
Q

Ag or Ab migrate up and down

A

Single dimension

17
Q

Ag diffuses in radial immunodiffusion producing _____

A

ring of precipitate

18
Q

Radial immunodiffusion is also known as

A

Mancini test

19
Q

Measurements are taken when the ring has stopped expanding

A

Mancini/Endpoint

20
Q

Measurement are taken while disc is still expanding

A

Kinetic Method

21
Q

Molecules with a net charge are separated when electric field is applied

A

Electrophoresis

Immunology & Serology (6 decks)

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