Flashcards in skin 17: trichinosis Deck (26):
trichinosis (*a reportable disease*) caused by ??
Trichinella spiralis, a Helminth parasite
Rarely, Trichinella pseudospiralis can also cause trichinosis.
There are many (10) species in the genus Trichinella that reflect 3 distinct sylvatic cycles
These species have different epidemiological patterns and pathogenic potentials e.g., some are highly resistant to freezing.
trichinosis high risk groups are mainly due to cultural/behavioral factors, mainly eating:
undercooked, raw animal flesh
pork, horse, wild/feral game meat.
Sporadic outbreaks due to contamination of food products. A butcher/food processor grinds up pork then fails to break down and properly clean the food grinding machinery.
Incidence of the genus Trichinella:
Almost worldwide distribution in feral animals (predominately omnivores & carnivores). Due to a broad-host range (virtually all warm-blooded animals).
In domestic swine (pig) and equine (horse) herds in the world: *T. spiralis* is dominant.
Incidence of trichinella infection or disease in humans is a reflection of ??
incidence of infection or disease in swine and horse herds.
Pathogenesis/Life cycle of T. spiralis (a Helminth/Nematode).
1. Flesh (striated muscle) eaten by carnivore or omnivore - pig, man, bears, etc
2. Larvae excyst in
the upper GI tract, due to
3. Larvae mature (2 d post-infection), adults mate while borrowed into the lining of the small intestine (S.I.)
4. Female borrows deeper into S.I. lining; releases larvae over 4-16w (begins 5d post-infection)
more Pathogenesis/Life cycle of T. spiralis (a Helminth/Nematode).
5. Larvae undergo several developmental cycles
6. First larvae enter the circulation, then migrate
into all body tissues (7-14d post-infection)
7. Only Larvae embed in
active, striated muscle elongate & encyst (21d post-infection)
most trichinella infections are ??
asymptomatic (probably due to consumption of low numbers of cysts).
trichinella Symptomatic infections: s/s are ??
*dose dependent*/depend on worm burden (numbers of ingested cysts).
due to *larval migration into tissue*, especially:
due to the *host immune response* to the larvae, which occur 7-21 days post-infection
Adults helminths (trichinella) cause s/s in the *GIT (Enteral phase)* by borrowing into mucosa of small intestine, causing:
nausea, vomiting, diarrhea, fever.
trichinella larvae cause s/s throughout the *body (Parenteral phase)* by:
entering the circulatory system->disseminating->exiting the circulatory system, damaging the circulatory system (trauma to the host circulatory system).
trichinella larvae also cause s/s throughout the *body (Parenteral phase)* by:
migrating through all body tissues/organs; (tissue migration occurs in
striated muscle and other tissue, most frequently in the organs with largest number of endarterioles) causing *damage to the tissue* (trauma to other host tissue): heart, kidney, lungs, CNS tissue
trichinella larvae attempt to encyst in all tissue but only succeed in ??
Unencysted larva die and the dead larvae and damaged host tissue must be cleared in all tissues they die in: how does this damage host??
Toxic compounds released during and after burrowing of larvae in body tissues (i.e. due to death of human cells and larvae) also damage the host.
Host immune response to trichinella produces ??
immunopathology (7-21d post infection); cause s/s *throughout the body (Parenteral phase)*:
-Allergic responses - Type I hypersensitivity.
-Antigen-antibody deposition vasculitis, Type III hypersensitivity.
trichinella larvae in striated muscle:
turns muscle cell into??
how does the host respond?
how long can incapsulated larvae remain viable?
-Phenotypically reprograms the muscle cell into a “nurse cell” i.e., incubator.
-Elongate and coil up;
Meanwhile, the nurse cells encapsulate themselves in collagen.
An intense inflammatory/host immune response (PMNs, eosinophils, lymphocytes) occurs, surrounds and finally calcify/encyst (6-18 m later) the nurse cell.
Encapsulated larvae can remain viable for years (up to 30 y).
trichinella larvae in striated muscle causes ?? to develop
T. spiralis-specific antibodies (IgA, IgM, IgG, esp. IgE) develop.
Antibodies plus eosinophils:
-mediate destruction of circulating larvae (antibody-dependent cell- mediated cytotoxity; ADCC).
-hasten expulsion of adult worm from the body in the feces.
Periorbital edema, Chemosis
which slowly subside, but can persist for up to 710 weeks post-infection.
s/s + history of eating raw or undercooked pork, feral animal flesh in last 3 w.
-Small biopsy specimen from deltoid or other muscle taken 3rd week post-infection or after will reveal larvae. Infected muscles lose their cross-striation, undergo basophilic degranulation.
more trichinosis dx
-Bentonite flocculation test (CDC) - utilizes Ag bound to bentonite clay to determine Ab levels.
-Indirect fluorescent antibody test (IFA), latex agglutination test, ELISA/EIA to detect T. spiralis-specific antibodies during the 1st week of infection.
trichinosis lab data
Serum enzyme levels (FYI Creatine phosphokinase, LDH) are determined to distinguish between heart and skeletal muscle damage. Increased serum markers are first detected during the 2nd week post-infection.
Eosinophila begins 2nd week of infection and persists until the infection resolves.
Supportive: mechanical ventilation.
Antihelminthics for trichinosis:
how do they work?
Benzimidazole derivatives (Albendazole, Albenzatm, mebendazole, [thiabendazole - no longer available due to side effects]
-Inhibit glucose uptake by blocking microtubule assembly.
-It is unclear which stages are affected by these drugs (i.e., only effective against larvae
in intestine before becoming the adult vs. only the adult worm [halt larval production]
vs. larvae in intestine and larvae in circulation).
In severely infected patient, antihelminthics can induce ??
a severe hypersensitivity
response and patient death.
Corticosteroids for trichinosis:
i.e. methylprednisolone to ameliorate allergic response during larval encystment.
-Cook pork or flesh of wild omnivore completely and until well-done (FYI achieve an internal temperature of 170oF [77oC] for 4 minutes).
-Irradiate pork before selling to the public.
-Cook garbage/animal feed before feeding pigs.
-Control rat infestations around pig farms.